Simultaneous analysis of amino and organic acids in extracts of plant leaves as tert-Butyldimethylsilyl derivatives by capillary gas chromatography

Z. Chen, P. Landman, Tim Colmer, M.A. Adams

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37 Citations (Scopus)

Abstract

The simultaneous determination of amino and organic acids in plant tissue extracts using capillary gas chromatography is described. Plant leaves were extracted in 5% (w/v) perchloric acid and neutralized extracts were purified using C-18 cartridges. The amino and organic acids in purified extracts mere then converted to tert-butyldimethylsilyl (TBDMS) derivatives prior to separation and detection by capillary gas chromatography (GC) with flame ionization detection. Conditions required for optimal derivatization were investigated. Amino and organic acids were readily converted to their TBDMS derivatives using N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide in dimethylformamide solvent 1:6 (v/v) with an average recovery of 90% and a reproducibility of about 5%. The characteristic [M-57] and [M-159] fragment ion of the TBDMS derivatives was confirmed using CC-MS, The proposed method was demonstrated by the determination of amino and organic acids in extracts of Acacia and Eucalyptus leaves, where detection limits were 1-20 ng. (C) 1998 Academic Press.
Original languageEnglish
Pages (from-to)203-211
JournalAnalytical Biochemistry
Volume259
DOIs
Publication statusPublished - 1998

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