[Truncated abstract] Helicobacter pylori infection is one of the most prevalent in the world causing a significant impact on human health. The infection can lead to various chronic gastric diseases including, gastritis, peptic ulcers and gastric carcinoma. Despite the availability and effectiveness of antimicrobial therapies, the cost of treatment and the emergence of resistant strains are leading to the consideration of alternative methods, including antibody therapy, to control the bacterium. Animal and human prophylactic studies have shown potential value of orally administered non-human antibodies against some human pathogens. Passive immunisation with orally administered bovine antibodies is a promising way to control H. pylori infection especially in developing countries. This thesis has aimed to assess the control of H. pylori infection by the development of whey protein concentrate prepared from the milk of immunised sheep as passive immunotherapy. A series of in vitro and in vivo experiments characterising the antibodies obtained after immunising sheep with various H. pylori antigens and investigating their efficacy to block certain virulence properties of H. pylori were conducted. The ultimate aim was to assess the efficacy of specific anti-H. pylori heterologous antibodies induced in sheep milk in preventing the infection. This approach was based on the hypothesis that antibodies of appropriate specificity for key virulence factors will effectively disarm the bacterium and thereby interfere with the colonisation process at the level of the gastric mucosa.
|Qualification||Doctor of Philosophy|
|Publication status||Unpublished - 2012|