TY - JOUR
T1 - Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson's Disease Patients
AU - Kurvits, Lille
AU - Reimann, Ene
AU - Kadastik-Eerme, Liis
AU - Truu, Laura
AU - Kingo, Kulli
AU - Erm, Triin
AU - Koks, Sulev
AU - Taba, Pille
AU - Planken, Anu
PY - 2019/1/29
Y1 - 2019/1/29
N2 - We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson's disease (PD) patients' peripheral tissues. We have previously shown that Saa-1 and -2 (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients' skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched controls. qRT-PCR analysis of PD skin demonstrated downregulation of Saa-1 and -2 genes in PD patients. However, the lowered amount of protein could not be visualized using immunohistochemistry, due to low quantity of SAA (Serum Amyloid Alpha, protein) in skin. Saa-1 and -2 expression levels in whole blood were below detection threshold based on RNA sequencing, however significantly lowered protein levels of SAA1/2 in PD patients' serum were shown with ELISA, implying that SAA is secreted into the blood. These results show that SAA is differentially expressed in the peripheral tissues of PD patients.
AB - We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson's disease (PD) patients' peripheral tissues. We have previously shown that Saa-1 and -2 (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients' skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched controls. qRT-PCR analysis of PD skin demonstrated downregulation of Saa-1 and -2 genes in PD patients. However, the lowered amount of protein could not be visualized using immunohistochemistry, due to low quantity of SAA (Serum Amyloid Alpha, protein) in skin. Saa-1 and -2 expression levels in whole blood were below detection threshold based on RNA sequencing, however significantly lowered protein levels of SAA1/2 in PD patients' serum were shown with ELISA, implying that SAA is secreted into the blood. These results show that SAA is differentially expressed in the peripheral tissues of PD patients.
KW - neurodegenarative disease
KW - serum amyloid A (SAA)
KW - Parkinson's disease (PD)
KW - intrinsically disordered protein (IDP)
KW - skin biopsy
KW - LEWY BODY
KW - EXPRESSION
KW - PROTEIN
KW - PATHOLOGY
KW - SYNUCLEIN
KW - DISORDER
KW - BRAIN
KW - MODEL
U2 - 10.3389/fnins.2019.00013
DO - 10.3389/fnins.2019.00013
M3 - Article
C2 - 30760975
VL - 13
JO - Frontiers in Neuroscience
JF - Frontiers in Neuroscience
SN - 1662-4548
IS - JAN
M1 - 13
ER -