Sequential monitoring of peripheral blood lymphocyte subsets in rats

S C Thompson, K M Bowen, R C Burton

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The methodology of enumeration of T cell subsets in humans is well established and has widespread clinical application. Sequential monitoring of lymphocyte subsets in small animals requires a consistent and reliable methodology for staining and enumerating lymphocyte populations. The optimal conditions for enumerating subclasses of rat peripheral blood lymphocytes with the Spectrum III flow cytometer are described. The sources of biological variation and technical error that were considered in optimizing the techniques and the limitations in applying them are discussed.

Original languageEnglish
Pages (from-to)184-93
Number of pages10
JournalCytometry
Volume7
Issue number2
DOIs
Publication statusPublished - Mar 1986

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Lymphocyte Subsets
Lymphocytes
T-Lymphocyte Subsets
Staining and Labeling
Population

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Thompson, S C ; Bowen, K M ; Burton, R C. / Sequential monitoring of peripheral blood lymphocyte subsets in rats. In: Cytometry. 1986 ; Vol. 7, No. 2. pp. 184-93.
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Sequential monitoring of peripheral blood lymphocyte subsets in rats. / Thompson, S C; Bowen, K M; Burton, R C.

In: Cytometry, Vol. 7, No. 2, 03.1986, p. 184-93.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sequential monitoring of peripheral blood lymphocyte subsets in rats

AU - Thompson, S C

AU - Bowen, K M

AU - Burton, R C

PY - 1986/3

Y1 - 1986/3

N2 - The methodology of enumeration of T cell subsets in humans is well established and has widespread clinical application. Sequential monitoring of lymphocyte subsets in small animals requires a consistent and reliable methodology for staining and enumerating lymphocyte populations. The optimal conditions for enumerating subclasses of rat peripheral blood lymphocytes with the Spectrum III flow cytometer are described. The sources of biological variation and technical error that were considered in optimizing the techniques and the limitations in applying them are discussed.

AB - The methodology of enumeration of T cell subsets in humans is well established and has widespread clinical application. Sequential monitoring of lymphocyte subsets in small animals requires a consistent and reliable methodology for staining and enumerating lymphocyte populations. The optimal conditions for enumerating subclasses of rat peripheral blood lymphocytes with the Spectrum III flow cytometer are described. The sources of biological variation and technical error that were considered in optimizing the techniques and the limitations in applying them are discussed.

KW - Animals

KW - Antibodies, Monoclonal

KW - Blood Preservation

KW - Cell Separation

KW - Flow Cytometry

KW - Leukocyte Count

KW - Lymphocytes/classification

KW - Male

KW - Monitoring, Physiologic/methods

KW - Rats

KW - Rats, Inbred Strains

KW - Time Factors

U2 - 10.1002/cyto.990070210

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M3 - Article

VL - 7

SP - 184

EP - 193

JO - Cytometry

JF - Cytometry

SN - 0196-4763

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ER -