TY - JOUR
T1 - Sequence diversification in recessive alleles of two host factor genes suggests adaptive selection for bymovirus resistance in cultivated barley from East Asia
AU - Yang, Ping
AU - Habekuß, Antje
AU - Hofinger, Bernhard J.
AU - Kanyuka, Kostya
AU - Kilian, Benjamin
AU - Graner, Andreas
AU - Ordon, Frank
AU - Stein, Nils
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Key message: Two distinct patterns of sequence diversity for the recessive alleles of two host factorsHvPDIL5-1andHvEIF4Eindicated the adaptive selection for bymovirus resistance in cultivated barley from East Asia. Abstract: Plant pathogens are constantly challenging plant fitness and driving resistance gene evolution in host species. Little is known about the evolution of sequence diversity in host recessive resistance genes that interact with plant viruses. Here, by combining previously published and newly generated targeted re-sequencing information, we systematically analyzed natural variation in a broad collection of wild (Hordeum spontaneum; Hs) and domesticated barleys (Hordeum vulgare; Hv) using the full-length coding sequence of the two host factor genes, HvPDIL5-1 and HvEIF4E, conferring recessive resistance to the agriculturally important Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV). Interestingly, two types of gene evolution conferred by sequence variation in domesticated barley, but not in wild barley were observed. Whereas resistance-conferring alleles of HvEIF4E exclusively contained non-synonymous amino acid substitutions (including in-frame sequence deletions and insertions), loss-of-function alleles were predominantly responsible for the HvPDIL5-1 conferred bymovirus resistance. A strong correlation between the geographic origin and the frequency of barley accessions carrying resistance-conferring alleles was evident for each of the two host factor genes, indicating adaptive selection for bymovirus resistance in cultivated barley from East Asia.
AB - Key message: Two distinct patterns of sequence diversity for the recessive alleles of two host factorsHvPDIL5-1andHvEIF4Eindicated the adaptive selection for bymovirus resistance in cultivated barley from East Asia. Abstract: Plant pathogens are constantly challenging plant fitness and driving resistance gene evolution in host species. Little is known about the evolution of sequence diversity in host recessive resistance genes that interact with plant viruses. Here, by combining previously published and newly generated targeted re-sequencing information, we systematically analyzed natural variation in a broad collection of wild (Hordeum spontaneum; Hs) and domesticated barleys (Hordeum vulgare; Hv) using the full-length coding sequence of the two host factor genes, HvPDIL5-1 and HvEIF4E, conferring recessive resistance to the agriculturally important Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV). Interestingly, two types of gene evolution conferred by sequence variation in domesticated barley, but not in wild barley were observed. Whereas resistance-conferring alleles of HvEIF4E exclusively contained non-synonymous amino acid substitutions (including in-frame sequence deletions and insertions), loss-of-function alleles were predominantly responsible for the HvPDIL5-1 conferred bymovirus resistance. A strong correlation between the geographic origin and the frequency of barley accessions carrying resistance-conferring alleles was evident for each of the two host factor genes, indicating adaptive selection for bymovirus resistance in cultivated barley from East Asia.
UR - http://www.scopus.com/inward/record.url?scp=84994474406&partnerID=8YFLogxK
U2 - 10.1007/s00122-016-2814-z
DO - 10.1007/s00122-016-2814-z
M3 - Article
C2 - 27830284
AN - SCOPUS:84994474406
SN - 0040-5752
VL - 130
SP - 331
EP - 344
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
IS - 2
ER -