Sequence differences between HLA B and TNF distinguish different MHC ancestral haplotypes

L.J. Abraham, C. Leelayuwat, G. Grimsley, M.A. Degli-Esposti, A. Mann, W.J. Zhang, Frank Christiansen, R.L. Dawkins

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Abstract

The HLA-B locus is extremely polymorphic. We have sequenced a region, CL, telomeric of HLA-B that also shows a high degree of allelic variation which we have shown previously by RFLP analysis. The polymorphism can be accounted for by sequence variation in duplicated, reiterated sequence elements called geometric elements. Comparison of the CL1 and CL2 sequences from the 57.1, 8.1, 18.2 and 7.1 ancestral haplotypes revealed that the lengths of the elements vary, both between the duplicated loci within a haplotype and between haplotypes, apparently because certain sequences are inserted or deleted. It is possible, using the polymerase chain reaction, to amplify these elements in genomic DNA from ancestral haplotypes for which sequence data of the CL region are not available and to obtain gel patterns which are characteristic of different ancestral haplotypes. The most striking feature of the data is the fact that the majority of the CL patterns are haplospecific; i.e. have a particular pattern that is unique for a particular ancestral haplotype and can be used to type these ancestral haplotypes. At least 12 different allelic patterns have been identified within a panel of 29 cell lines representing 16 ancestral haplotypes. For these 16 ancestral haplotypes, all examples of each haplotype have the same CL pattern. The haplotypic nature of the patterns confirms that ancestral haplotypes are conserved chromosomal segments and that coding and non-coding sequences are identical by descent from a remote ancestor.
Original languageEnglish
Pages (from-to)117-117
JournalTissue Antigens
Volume39
Issue number3
DOIs
Publication statusPublished - 1992

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HLA-B Antigens
Haplotypes
Polymerase chain reaction
Polymorphism
Gels
Cells
DNA
Restriction Fragment Length Polymorphisms

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Abraham, L. J., Leelayuwat, C., Grimsley, G., Degli-Esposti, M. A., Mann, A., Zhang, W. J., ... Dawkins, R. L. (1992). Sequence differences between HLA B and TNF distinguish different MHC ancestral haplotypes. Tissue Antigens, 39(3), 117-117. https://doi.org/10.1111/j.1399-0039.1992.tb01920.x
Abraham, L.J. ; Leelayuwat, C. ; Grimsley, G. ; Degli-Esposti, M.A. ; Mann, A. ; Zhang, W.J. ; Christiansen, Frank ; Dawkins, R.L. / Sequence differences between HLA B and TNF distinguish different MHC ancestral haplotypes. In: Tissue Antigens. 1992 ; Vol. 39, No. 3. pp. 117-117.
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Abraham, LJ, Leelayuwat, C, Grimsley, G, Degli-Esposti, MA, Mann, A, Zhang, WJ, Christiansen, F & Dawkins, RL 1992, 'Sequence differences between HLA B and TNF distinguish different MHC ancestral haplotypes' Tissue Antigens, vol. 39, no. 3, pp. 117-117. https://doi.org/10.1111/j.1399-0039.1992.tb01920.x

Sequence differences between HLA B and TNF distinguish different MHC ancestral haplotypes. / Abraham, L.J.; Leelayuwat, C.; Grimsley, G.; Degli-Esposti, M.A.; Mann, A.; Zhang, W.J.; Christiansen, Frank; Dawkins, R.L.

In: Tissue Antigens, Vol. 39, No. 3, 1992, p. 117-117.

Research output: Contribution to journalArticle

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AU - Abraham, L.J.

AU - Leelayuwat, C.

AU - Grimsley, G.

AU - Degli-Esposti, M.A.

AU - Mann, A.

AU - Zhang, W.J.

AU - Christiansen, Frank

AU - Dawkins, R.L.

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N2 - The HLA-B locus is extremely polymorphic. We have sequenced a region, CL, telomeric of HLA-B that also shows a high degree of allelic variation which we have shown previously by RFLP analysis. The polymorphism can be accounted for by sequence variation in duplicated, reiterated sequence elements called geometric elements. Comparison of the CL1 and CL2 sequences from the 57.1, 8.1, 18.2 and 7.1 ancestral haplotypes revealed that the lengths of the elements vary, both between the duplicated loci within a haplotype and between haplotypes, apparently because certain sequences are inserted or deleted. It is possible, using the polymerase chain reaction, to amplify these elements in genomic DNA from ancestral haplotypes for which sequence data of the CL region are not available and to obtain gel patterns which are characteristic of different ancestral haplotypes. The most striking feature of the data is the fact that the majority of the CL patterns are haplospecific; i.e. have a particular pattern that is unique for a particular ancestral haplotype and can be used to type these ancestral haplotypes. At least 12 different allelic patterns have been identified within a panel of 29 cell lines representing 16 ancestral haplotypes. For these 16 ancestral haplotypes, all examples of each haplotype have the same CL pattern. The haplotypic nature of the patterns confirms that ancestral haplotypes are conserved chromosomal segments and that coding and non-coding sequences are identical by descent from a remote ancestor.

AB - The HLA-B locus is extremely polymorphic. We have sequenced a region, CL, telomeric of HLA-B that also shows a high degree of allelic variation which we have shown previously by RFLP analysis. The polymorphism can be accounted for by sequence variation in duplicated, reiterated sequence elements called geometric elements. Comparison of the CL1 and CL2 sequences from the 57.1, 8.1, 18.2 and 7.1 ancestral haplotypes revealed that the lengths of the elements vary, both between the duplicated loci within a haplotype and between haplotypes, apparently because certain sequences are inserted or deleted. It is possible, using the polymerase chain reaction, to amplify these elements in genomic DNA from ancestral haplotypes for which sequence data of the CL region are not available and to obtain gel patterns which are characteristic of different ancestral haplotypes. The most striking feature of the data is the fact that the majority of the CL patterns are haplospecific; i.e. have a particular pattern that is unique for a particular ancestral haplotype and can be used to type these ancestral haplotypes. At least 12 different allelic patterns have been identified within a panel of 29 cell lines representing 16 ancestral haplotypes. For these 16 ancestral haplotypes, all examples of each haplotype have the same CL pattern. The haplotypic nature of the patterns confirms that ancestral haplotypes are conserved chromosomal segments and that coding and non-coding sequences are identical by descent from a remote ancestor.

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JO - Tissue Antigens

JF - Tissue Antigens

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Abraham LJ, Leelayuwat C, Grimsley G, Degli-Esposti MA, Mann A, Zhang WJ et al. Sequence differences between HLA B and TNF distinguish different MHC ancestral haplotypes. Tissue Antigens. 1992;39(3):117-117. https://doi.org/10.1111/j.1399-0039.1992.tb01920.x