Separation of amino acids in plant tissue extracts by capillary zone electrophoresis with indirect UV detection using aromatic carboxylates as background electrolytes.

Z. Chen, C.R. Warren, M.A. Adams

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Amino acids in extracts of plant tissue were separated and detected by capillary zone electrophoresis (CZE) with indirect UV detection. Various aromatic carboxylates such as salicylate, benzoate, phthalate and trimellitate were investigated as background electrolytes (BGEs). A BGE of benzoate gave the best resolution and detector response. Amino acids were separated at a highly alkaline pH to charge amino acids negatively. Separataion was achieved by the co-electroosmotic flow (Co-EOF) by the addition of the cationic surfactant myristyltrimethyl-ammonium bromide (MTAB) to the electrolyte. The condtions affecting the separation of amino acids, including electrolyte pH, concentrations of both benzoate and MTAB, were investigated and optimised. Separation of a mixture of 17 amino acids at pH 11.20 with indirect UV detection at 225 nm was achieved with a BGE of 10 mM benzoate containing 1.0 mM MTAB at pH of 11.20. Detection limits ranged between 10 and 50 mu M The proposed method was demonstrated by the determination of amino acids in extracts of Eucalypt leaves with direct injection of samples.
Original languageEnglish
Pages (from-to)180-186
JournalChromatographia
Volume51
Issue number3/4
DOIs
Publication statusPublished - 2000

    Fingerprint

Cite this