1. We investigated the feasibility of training male ostriches to ejaculate into an artificial cloaca ( AC) using a teaser female or a dummy. 2. Male ostriches develop desirable behaviour patterns that allow them to be trained to the teaser and the dummy female. Training success can be high as both methods rely on natural stimulation and voluntary ejaculation into the AC provided suitable individuals are identified. 3. The key factors to successful training appear to be: the teaser method - temperament of male ostriches, the crouching behaviour of the female ostriches for the semen collector and acceptance of the collector by the male; dummy method - courtship of humans, learning ability of ostriches to habituate and then to mount a dummy. 4. Both methods are reliable, yield ejaculates of high quality and give reproducible results. The ejaculate volume was 1 center dot 09 center dot 0 center dot 13 ml, the concentration of spermatozoa 4 center dot 21 center dot 0 center dot 27 center dot 10(9)/ ml, the total number of spermatozoa 4 center dot 67 +/- 0 center dot 62 x 10(9) and motility 4 center dot 3 +/- 0 center dot 1. 5. Commercial ostrich production that relies on natural mating can take advantage of those methods to develop artificial insemination technologies.