Seed provenance determines germination responses of Rumex crispus (L.) under water stress and nutrient availability

María Pérez-Fernández, Carole P. Elliott, Alex Valentine, José Antonio Oyola

Research output: Contribution to journalArticle

Abstract

Aims: Seeds of Rumex crispus from six provenances were studied in relation to their germination under drought and presence of nitrogen in the germination and emergence media. We also investigated whether adaptation to soil increases the ability of the species to colonize and establish in contrasting environments along a longitudinal gradient in western Spain by means of a reciprocal transplantation experiment. Methods: We conducted a germination trial in the lab to test for the germination responses to water scarcity along a polyethylene glycol gradient and to varying concentrations of nitrogen compounds. Simultaneously reciprocal transplantations experiment was conducted, where seeds from six provenances were grown in the soils from the very same provenances. Seedling emergence, survivorship and fitness-related variables were measured in all plots. Important Findings: We found that R. crispus has a cold-stratification requirement that enhances its germination. Significant differences between the six provenances were detected for time-to-germination, total seedling emergence, plant mortality and reproductive effort in all the experiments. The differences between provenances with respect to germination were confirmed by the significant statistical analyses of the variance, thus providing evidence that seeds from parent plants grown in different environmental conditions have an intrinsically different abilities to germinate and establish. Soil nitrogen content where seed germination and seedlings establish also play an important role in their performance in terms of survivorship and reproduction, being the higher levels of inorganic nitrogen and of microbial biomass those that increased biomass production, enhanced inflorescence formation and reduced plant mortality. We conclude that one of the main reasons for the spread and maintenance of R. crispus would be the increased levels of nitrogen in agricultural soils.

Original languageEnglish
Pages (from-to)949-961
Number of pages13
JournalJournal of Plant Ecology
Volume12
Issue number6
DOIs
Publication statusPublished - 5 Jun 2019

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Rumex crispus
nutrient availability
water stress
provenance
germination
seed
seeds
seedling emergence
transplantation
survivorship
nitrogen
survival rate
soil
seed stratification
water shortages
longitudinal gradient
mortality
nitrogen compounds
reproductive effort
polyethylene glycol

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Pérez-Fernández, María ; Elliott, Carole P. ; Valentine, Alex ; Oyola, José Antonio. / Seed provenance determines germination responses of Rumex crispus (L.) under water stress and nutrient availability. In: Journal of Plant Ecology. 2019 ; Vol. 12, No. 6. pp. 949-961.
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abstract = "Aims: Seeds of Rumex crispus from six provenances were studied in relation to their germination under drought and presence of nitrogen in the germination and emergence media. We also investigated whether adaptation to soil increases the ability of the species to colonize and establish in contrasting environments along a longitudinal gradient in western Spain by means of a reciprocal transplantation experiment. Methods: We conducted a germination trial in the lab to test for the germination responses to water scarcity along a polyethylene glycol gradient and to varying concentrations of nitrogen compounds. Simultaneously reciprocal transplantations experiment was conducted, where seeds from six provenances were grown in the soils from the very same provenances. Seedling emergence, survivorship and fitness-related variables were measured in all plots. Important Findings: We found that R. crispus has a cold-stratification requirement that enhances its germination. Significant differences between the six provenances were detected for time-to-germination, total seedling emergence, plant mortality and reproductive effort in all the experiments. The differences between provenances with respect to germination were confirmed by the significant statistical analyses of the variance, thus providing evidence that seeds from parent plants grown in different environmental conditions have an intrinsically different abilities to germinate and establish. Soil nitrogen content where seed germination and seedlings establish also play an important role in their performance in terms of survivorship and reproduction, being the higher levels of inorganic nitrogen and of microbial biomass those that increased biomass production, enhanced inflorescence formation and reduced plant mortality. We conclude that one of the main reasons for the spread and maintenance of R. crispus would be the increased levels of nitrogen in agricultural soils.",
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Seed provenance determines germination responses of Rumex crispus (L.) under water stress and nutrient availability. / Pérez-Fernández, María; Elliott, Carole P.; Valentine, Alex; Oyola, José Antonio.

In: Journal of Plant Ecology, Vol. 12, No. 6, 05.06.2019, p. 949-961.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Seed provenance determines germination responses of Rumex crispus (L.) under water stress and nutrient availability

AU - Pérez-Fernández, María

AU - Elliott, Carole P.

AU - Valentine, Alex

AU - Oyola, José Antonio

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AB - Aims: Seeds of Rumex crispus from six provenances were studied in relation to their germination under drought and presence of nitrogen in the germination and emergence media. We also investigated whether adaptation to soil increases the ability of the species to colonize and establish in contrasting environments along a longitudinal gradient in western Spain by means of a reciprocal transplantation experiment. Methods: We conducted a germination trial in the lab to test for the germination responses to water scarcity along a polyethylene glycol gradient and to varying concentrations of nitrogen compounds. Simultaneously reciprocal transplantations experiment was conducted, where seeds from six provenances were grown in the soils from the very same provenances. Seedling emergence, survivorship and fitness-related variables were measured in all plots. Important Findings: We found that R. crispus has a cold-stratification requirement that enhances its germination. Significant differences between the six provenances were detected for time-to-germination, total seedling emergence, plant mortality and reproductive effort in all the experiments. The differences between provenances with respect to germination were confirmed by the significant statistical analyses of the variance, thus providing evidence that seeds from parent plants grown in different environmental conditions have an intrinsically different abilities to germinate and establish. Soil nitrogen content where seed germination and seedlings establish also play an important role in their performance in terms of survivorship and reproduction, being the higher levels of inorganic nitrogen and of microbial biomass those that increased biomass production, enhanced inflorescence formation and reduced plant mortality. We conclude that one of the main reasons for the spread and maintenance of R. crispus would be the increased levels of nitrogen in agricultural soils.

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KW - latitudinal gradient

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