Sample storage conditions alter colonisation structures of arbuscular mycorrhizal fungi and, particularly, fine root endophyte

Suzanne Orchard, R. J. Standish, D. Nicol, Ian A. Dickie, M. H. Ryan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background and Aims: The structures of arbuscular mycorrhizal (AM) fungi (hyphae, arbuscules, vesicles, spores) are used to make inferences about fungal activity based on stored samples, yet the impact of storage method has not been quantified, despite known effects of temperature and host condition on AM fungal colonisation. Methods: We measured how four storage treatments (cool or ambient conditions, with and without plant shoots attached, i.e. n = four treatment combinations) affected AM fungal colonisation of subterranean clover (Trifolium subterraneum L.) after 0, 2, 6 and 10 days of storage. Roots were assessed for colonisation of fine root endophyte and coarse AM fungi. Results: For coarse AM fungi, total colonisation was unaffected, but arbuscules were reduced at Day 6 and increased again by Day 10, except Ambient-Minus-Shoots. There was a loss of vesicles in all treatments at Day 2, and an increase in spore number at Day 6 within Cool-Plus-Shoots. In contrast, for fine root endophyte, total colonisation was greatly reduced at Day 6 but increased again at Day 10, in all except the Cool-Plus-Shoots treatment. Conclusions: Our data demonstrate that AM fungal activity is not suspended in commonly used plant storage conditions. Storage method and time impacted AM fungal colonisation, particularly for fine root endophyte. We recommend samples are processed within 2 days of harvest.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalPlant and Soil
Volume412
Issue number1-2
DOIs
Publication statusPublished - 1 Mar 2017

Fingerprint Dive into the research topics of 'Sample storage conditions alter colonisation structures of arbuscular mycorrhizal fungi and, particularly, fine root endophyte'. Together they form a unique fingerprint.

Cite this