Salt sensitivity in chickpea (Cicer arietinumL.): Ions in reproductive tissues and yield components in contrasting genotypes

Lukasz Kotula, Hammad Khan, John Quealy, Neil Turner, V. Vadez, Kadambot Siddique, Peta Clode, Tim Colmer

Research output: Contribution to journal › Article

26 Citations (Scopus)

Abstract

© 2015 John Wiley & Sons Ltd. The reproductive phase in chickpea (Cicer arietinumL.) is affected by salinity, but little is known about the underlying cause. We investigated whether high concentrations of Na+ and Cl- in the reproductive structures influence reproductive processes. Chickpea genotypes contrasting in tolerance were subjected to 0, 35 or 50mm NaCl applied to soil in pots. Flower production and abortion, pod number, percentage of empty pods, seed number and size were evaluated. The concentrations of Na+, K+ and Cl- were measured in various plant tissues and, using X-ray microanalysis, in specific cells of developing reproductive structures. Genotypic variation in reproductive success measured as seed yield in saline conditions was associated with better maintenance of flower production and higher numbers of filled pods (and thus seed number), whereas seed size decreased in all genotypes. Despite the variation in reproductive success, the accumulation of Na+ and Cl- in the early reproductive tissues of developing pods did not differ between a tolerant (Genesis836) and a sensitive (Rupali) genotype. Similarly, salinity tolerance was not associated with the accumulation of salt ions in leaves at the time of reproduction or in seeds at maturity. We studied whether the adverse effects of salinity on reproductive processes in chickpea are associated with ion accumulation in reproductive tissues. Quantitative X-ray microanalysis was used to evaluate the concentrations of Na, K and Cl in specific cells of developing reproductive organs of two genotypes of contrasting salt tolerance. Despite large genotypic differences for seed yield of plants in saline soil, Na, K and Cl concentrations in developing ovules and pod walls were similar for the two genotypes. Although Na concentrations had increased in these early reproductive tissues the concentrations were relatively low. Na had also increased in mature seeds, but this did not impact their subsequent germination.
Original languageEnglish
Pages (from-to)1565-1577
JournalPlant, Cell and Environment
Volume38
Issue number8
DOIs
Publication statusPublished - 2015

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Cicer
yield components
Seeds
Salts
Genotype
Ions
pods
ions
salts
genotype
seeds
Salinity
salinity
Electron Probe Microanalysis
seed yield
X-radiation
flowers
abortion (plants)
saline soils
Soil

Cite this

Kotula, Lukasz ; Khan, Hammad ; Quealy, John ; Turner, Neil ; Vadez, V. ; Siddique, Kadambot ; Clode, Peta ; Colmer, Tim. / Salt sensitivity in chickpea (Cicer arietinumL.): Ions in reproductive tissues and yield components in contrasting genotypes. In: Plant, Cell and Environment. 2015 ; Vol. 38, No. 8. pp. 1565-1577.
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abstract = "{\circledC} 2015 John Wiley & Sons Ltd. The reproductive phase in chickpea (Cicer arietinumL.) is affected by salinity, but little is known about the underlying cause. We investigated whether high concentrations of Na+ and Cl- in the reproductive structures influence reproductive processes. Chickpea genotypes contrasting in tolerance were subjected to 0, 35 or 50mm NaCl applied to soil in pots. Flower production and abortion, pod number, percentage of empty pods, seed number and size were evaluated. The concentrations of Na+, K+ and Cl- were measured in various plant tissues and, using X-ray microanalysis, in specific cells of developing reproductive structures. Genotypic variation in reproductive success measured as seed yield in saline conditions was associated with better maintenance of flower production and higher numbers of filled pods (and thus seed number), whereas seed size decreased in all genotypes. Despite the variation in reproductive success, the accumulation of Na+ and Cl- in the early reproductive tissues of developing pods did not differ between a tolerant (Genesis836) and a sensitive (Rupali) genotype. Similarly, salinity tolerance was not associated with the accumulation of salt ions in leaves at the time of reproduction or in seeds at maturity. We studied whether the adverse effects of salinity on reproductive processes in chickpea are associated with ion accumulation in reproductive tissues. Quantitative X-ray microanalysis was used to evaluate the concentrations of Na, K and Cl in specific cells of developing reproductive organs of two genotypes of contrasting salt tolerance. Despite large genotypic differences for seed yield of plants in saline soil, Na, K and Cl concentrations in developing ovules and pod walls were similar for the two genotypes. Although Na concentrations had increased in these early reproductive tissues the concentrations were relatively low. Na had also increased in mature seeds, but this did not impact their subsequent germination.",
author = "Lukasz Kotula and Hammad Khan and John Quealy and Neil Turner and V. Vadez and Kadambot Siddique and Peta Clode and Tim Colmer",
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Kotula, L, Khan, H, Quealy, J, Turner, N, Vadez, V, Siddique, K, Clode, P & Colmer, T 2015, 'Salt sensitivity in chickpea (Cicer arietinumL.): Ions in reproductive tissues and yield components in contrasting genotypes' Plant, Cell and Environment, vol. 38, no. 8, pp. 1565-1577. https://doi.org/10.1111/pce.12506

Salt sensitivity in chickpea (Cicer arietinumL.): Ions in reproductive tissues and yield components in contrasting genotypes. / Kotula, Lukasz; Khan, Hammad; Quealy, John; Turner, Neil; Vadez, V.; Siddique, Kadambot; Clode, Peta; Colmer, Tim.

In: Plant, Cell and Environment, Vol. 38, No. 8, 2015, p. 1565-1577.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Salt sensitivity in chickpea (Cicer arietinumL.): Ions in reproductive tissues and yield components in contrasting genotypes

AU - Kotula, Lukasz

AU - Khan, Hammad

AU - Quealy, John

AU - Turner, Neil

AU - Vadez, V.

AU - Siddique, Kadambot

AU - Clode, Peta

AU - Colmer, Tim

PY - 2015

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N2 - © 2015 John Wiley & Sons Ltd. The reproductive phase in chickpea (Cicer arietinumL.) is affected by salinity, but little is known about the underlying cause. We investigated whether high concentrations of Na+ and Cl- in the reproductive structures influence reproductive processes. Chickpea genotypes contrasting in tolerance were subjected to 0, 35 or 50mm NaCl applied to soil in pots. Flower production and abortion, pod number, percentage of empty pods, seed number and size were evaluated. The concentrations of Na+, K+ and Cl- were measured in various plant tissues and, using X-ray microanalysis, in specific cells of developing reproductive structures. Genotypic variation in reproductive success measured as seed yield in saline conditions was associated with better maintenance of flower production and higher numbers of filled pods (and thus seed number), whereas seed size decreased in all genotypes. Despite the variation in reproductive success, the accumulation of Na+ and Cl- in the early reproductive tissues of developing pods did not differ between a tolerant (Genesis836) and a sensitive (Rupali) genotype. Similarly, salinity tolerance was not associated with the accumulation of salt ions in leaves at the time of reproduction or in seeds at maturity. We studied whether the adverse effects of salinity on reproductive processes in chickpea are associated with ion accumulation in reproductive tissues. Quantitative X-ray microanalysis was used to evaluate the concentrations of Na, K and Cl in specific cells of developing reproductive organs of two genotypes of contrasting salt tolerance. Despite large genotypic differences for seed yield of plants in saline soil, Na, K and Cl concentrations in developing ovules and pod walls were similar for the two genotypes. Although Na concentrations had increased in these early reproductive tissues the concentrations were relatively low. Na had also increased in mature seeds, but this did not impact their subsequent germination.

AB - © 2015 John Wiley & Sons Ltd. The reproductive phase in chickpea (Cicer arietinumL.) is affected by salinity, but little is known about the underlying cause. We investigated whether high concentrations of Na+ and Cl- in the reproductive structures influence reproductive processes. Chickpea genotypes contrasting in tolerance were subjected to 0, 35 or 50mm NaCl applied to soil in pots. Flower production and abortion, pod number, percentage of empty pods, seed number and size were evaluated. The concentrations of Na+, K+ and Cl- were measured in various plant tissues and, using X-ray microanalysis, in specific cells of developing reproductive structures. Genotypic variation in reproductive success measured as seed yield in saline conditions was associated with better maintenance of flower production and higher numbers of filled pods (and thus seed number), whereas seed size decreased in all genotypes. Despite the variation in reproductive success, the accumulation of Na+ and Cl- in the early reproductive tissues of developing pods did not differ between a tolerant (Genesis836) and a sensitive (Rupali) genotype. Similarly, salinity tolerance was not associated with the accumulation of salt ions in leaves at the time of reproduction or in seeds at maturity. We studied whether the adverse effects of salinity on reproductive processes in chickpea are associated with ion accumulation in reproductive tissues. Quantitative X-ray microanalysis was used to evaluate the concentrations of Na, K and Cl in specific cells of developing reproductive organs of two genotypes of contrasting salt tolerance. Despite large genotypic differences for seed yield of plants in saline soil, Na, K and Cl concentrations in developing ovules and pod walls were similar for the two genotypes. Although Na concentrations had increased in these early reproductive tissues the concentrations were relatively low. Na had also increased in mature seeds, but this did not impact their subsequent germination.

U2 - 10.1111/pce.12506

DO - 10.1111/pce.12506

M3 - Article

VL - 38

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EP - 1577

JO - Plant, Cell and Environment.

JF - Plant, Cell and Environment.

SN - 0140-7791

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Kotula L, Khan H, Quealy J, Turner N, Vadez V, Siddique K et al. Salt sensitivity in chickpea (Cicer arietinumL.): Ions in reproductive tissues and yield components in contrasting genotypes. Plant, Cell and Environment. 2015;38(8):1565-1577. https://doi.org/10.1111/pce.12506

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