Salidroside promotes rat spinal cord injury recovery by inhibiting inflammatory cytokine expression and NF-κB and MAPK signaling pathways

Yiji Su, Shaohui Zong, Chengming Wei, Fangming Song, Haotian Feng, An Qin, Zhen Lian, Fangsheng Fu, Siyuan Shao, Fang Fang, Tailai Wu, Jiake Xu, Qian Liu, Jinmin Zhao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Spinal cord injury (SCI) is a public health problem in the world. The SCI usually triggers an excessive inflammatory response that brings about a secondary tissue wreck leading to further cellular and organ dysfunction. Hence, there is great potential of reducing inflammation for therapeutic strategies of SCI. In this study, we aim to investigate if Salidroside (SAD) exerts an anti-inflammatory effect and promotes recovery of motor function on SCI through suppressing nuclear factor-κB (NF-κB) and the mitogen-activated protein kinase (MAPK) pathways. In vitro, real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were used to examine the inhibitory effect of SAD on the expression and release of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) activated by lipopolysaccharide (LPS) in astrocytes. In addition, SAD was found to inhibit NF-κB, p38 and extracellular-regulated protein kinases (ERK) signaling pathways by western blot analysis. Further, in vivo study showed that SAD was able to improve hind limb motor function and reduce tissue damage accompanied by the suppressed expression of inflammatory cytokines IL-1β, IL-6, and TNF-α. Overall, SAD could reduce the inflammatory response and promote motor function recovery in rats after SCI by inhibiting NF-κB, p38, and ERK signaling pathways.

Original languageEnglish
Pages (from-to)14259-14269
Number of pages11
JournalJournal of Cellular Physiology
Volume234
Issue number8
DOIs
Publication statusPublished - 1 Aug 2019

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Mitogen-Activated Protein Kinases
Spinal Cord Injuries
Rats
Cytokines
Recovery
Recovery of Function
Interleukin-1
Protein Kinases
Interleukin-6
Tumor Necrosis Factor-alpha
Tissue
Immunosorbents
Polymerase chain reaction
Public health
Medical problems
Astrocytes
Lipopolysaccharides
Real-Time Polymerase Chain Reaction
Assays
Anti-Inflammatory Agents

Cite this

Su, Yiji ; Zong, Shaohui ; Wei, Chengming ; Song, Fangming ; Feng, Haotian ; Qin, An ; Lian, Zhen ; Fu, Fangsheng ; Shao, Siyuan ; Fang, Fang ; Wu, Tailai ; Xu, Jiake ; Liu, Qian ; Zhao, Jinmin. / Salidroside promotes rat spinal cord injury recovery by inhibiting inflammatory cytokine expression and NF-κB and MAPK signaling pathways. In: Journal of Cellular Physiology. 2019 ; Vol. 234, No. 8. pp. 14259-14269.
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abstract = "Spinal cord injury (SCI) is a public health problem in the world. The SCI usually triggers an excessive inflammatory response that brings about a secondary tissue wreck leading to further cellular and organ dysfunction. Hence, there is great potential of reducing inflammation for therapeutic strategies of SCI. In this study, we aim to investigate if Salidroside (SAD) exerts an anti-inflammatory effect and promotes recovery of motor function on SCI through suppressing nuclear factor-κB (NF-κB) and the mitogen-activated protein kinase (MAPK) pathways. In vitro, real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were used to examine the inhibitory effect of SAD on the expression and release of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) activated by lipopolysaccharide (LPS) in astrocytes. In addition, SAD was found to inhibit NF-κB, p38 and extracellular-regulated protein kinases (ERK) signaling pathways by western blot analysis. Further, in vivo study showed that SAD was able to improve hind limb motor function and reduce tissue damage accompanied by the suppressed expression of inflammatory cytokines IL-1β, IL-6, and TNF-α. Overall, SAD could reduce the inflammatory response and promote motor function recovery in rats after SCI by inhibiting NF-κB, p38, and ERK signaling pathways.",
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Salidroside promotes rat spinal cord injury recovery by inhibiting inflammatory cytokine expression and NF-κB and MAPK signaling pathways. / Su, Yiji; Zong, Shaohui; Wei, Chengming; Song, Fangming; Feng, Haotian; Qin, An; Lian, Zhen; Fu, Fangsheng; Shao, Siyuan; Fang, Fang; Wu, Tailai; Xu, Jiake; Liu, Qian; Zhao, Jinmin.

In: Journal of Cellular Physiology, Vol. 234, No. 8, 01.08.2019, p. 14259-14269.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Salidroside promotes rat spinal cord injury recovery by inhibiting inflammatory cytokine expression and NF-κB and MAPK signaling pathways

AU - Su, Yiji

AU - Zong, Shaohui

AU - Wei, Chengming

AU - Song, Fangming

AU - Feng, Haotian

AU - Qin, An

AU - Lian, Zhen

AU - Fu, Fangsheng

AU - Shao, Siyuan

AU - Fang, Fang

AU - Wu, Tailai

AU - Xu, Jiake

AU - Liu, Qian

AU - Zhao, Jinmin

PY - 2019/8/1

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N2 - Spinal cord injury (SCI) is a public health problem in the world. The SCI usually triggers an excessive inflammatory response that brings about a secondary tissue wreck leading to further cellular and organ dysfunction. Hence, there is great potential of reducing inflammation for therapeutic strategies of SCI. In this study, we aim to investigate if Salidroside (SAD) exerts an anti-inflammatory effect and promotes recovery of motor function on SCI through suppressing nuclear factor-κB (NF-κB) and the mitogen-activated protein kinase (MAPK) pathways. In vitro, real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were used to examine the inhibitory effect of SAD on the expression and release of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) activated by lipopolysaccharide (LPS) in astrocytes. In addition, SAD was found to inhibit NF-κB, p38 and extracellular-regulated protein kinases (ERK) signaling pathways by western blot analysis. Further, in vivo study showed that SAD was able to improve hind limb motor function and reduce tissue damage accompanied by the suppressed expression of inflammatory cytokines IL-1β, IL-6, and TNF-α. Overall, SAD could reduce the inflammatory response and promote motor function recovery in rats after SCI by inhibiting NF-κB, p38, and ERK signaling pathways.

AB - Spinal cord injury (SCI) is a public health problem in the world. The SCI usually triggers an excessive inflammatory response that brings about a secondary tissue wreck leading to further cellular and organ dysfunction. Hence, there is great potential of reducing inflammation for therapeutic strategies of SCI. In this study, we aim to investigate if Salidroside (SAD) exerts an anti-inflammatory effect and promotes recovery of motor function on SCI through suppressing nuclear factor-κB (NF-κB) and the mitogen-activated protein kinase (MAPK) pathways. In vitro, real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were used to examine the inhibitory effect of SAD on the expression and release of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) activated by lipopolysaccharide (LPS) in astrocytes. In addition, SAD was found to inhibit NF-κB, p38 and extracellular-regulated protein kinases (ERK) signaling pathways by western blot analysis. Further, in vivo study showed that SAD was able to improve hind limb motor function and reduce tissue damage accompanied by the suppressed expression of inflammatory cytokines IL-1β, IL-6, and TNF-α. Overall, SAD could reduce the inflammatory response and promote motor function recovery in rats after SCI by inhibiting NF-κB, p38, and ERK signaling pathways.

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KW - inflammatory cytokines

KW - lipopolysaccharide

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