S100A8 Induction in Keratinocytes by Ultraviolet A Irradiation Is Dependent on Reactive Oxygen Intermediates

Michele A. Grimbaldeston, Carolyn L. Geczy, Nicodemus Tedla, John J. Finlay-Jones, Prue H. Hart

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67 Citations (Scopus)


Cutaneous exposure to ultraviolet (UV) A (320-400 nm) results in the formation of damaging reactive oxygen intermediates, which are implicated as mediators of DNA damage, apoptosis, and photoaging. S100A8 is a low-molecular-weight calcium-binding protein, highly sensitive to oxidation. In this study, UVA-induced S100A8 expression by keratinocytes was investigated. UVA (50-100 kJ per m2) strongly induced S100A8 in differentiated keratinocytes in the epidermis of BALB/c mice. Similarly, S100A8 mRNA and monomeric protein were significantly upregulated in PAM212 cells (a murine keratinocyte cell line) in response to 10 kJ per m2 UVA 24 h after irradiation. Although S100A9 associates with S100A8 in neutrophils and abnormally differentiated keratinocytes (human psoriasis), in this study it was not coinduced with keratinocyte S100A8. Dorsal application of 4-hydroxy-tempo (a superoxide dismutase-mimicking agent) to mice concentrationdependently reduced UVA-induced S100A8 expression. Incubation of PAM212 cells with superoxide dismutase and catalase during UVA irradiation also abrogated S100A8 induction. These results suggest that UVA-induced S100A8 is expressed by keratinocytes in response to generation of reactive oxygen intermediates.

Original languageEnglish
Pages (from-to)1168-1174
Number of pages7
JournalJournal of Investigative Dermatology
Issue number5
Publication statusPublished - 1 Jan 2003
Externally publishedYes


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