RU486 inhibits ovulation, fertilization and early embryonic development in rabbits: in vivo and in vitro studies

S.H. Chen; Arunasalam Dharmarajan; E.E. Wallach; C. Mastroyannis

RU486 inhibits ovulation, fertilization and early embryonic development in rabbits: in vivo and in vitro studies

S.H. Chen, Arunasalam Dharmarajan, E.E. Wallach, C. Mastroyannis

Research output: Contribution to journal › Article

Abstract

Objective: To clarify the role of P in ovulation, fertilization, and early embryonic development using RU486, a potent P receptor blocker.Design: Ovulatory efficiency, IVF, and early embryonic development were evaluated after RU486 administration in vivo and in vitro.Setting: Research laboratory of a university hospital.Participants: Mature male and female New Zealand white rabbits.Interventions: Animals were treated with RU486 or vehicle for 3 days before hCG-induced ovulation. Ovaries treated with hCG to induce ovulation were perfused for 6 hours in vitro with RU486 or vehicle. In vitro fertilization was performed in the presence or absence of RU486.Main Outcome Measures: The percentage of mature follicles ovulating (ovulatory efficiency) was determined after in vivo and in vitro treatment. Fertilization, morula, and blastocyst development were evaluated every 24 hours for 120 hours.Results: RU486 significantly inhibited ovulation, fertilization, and early embryonic development.Conclusions: Progesterone plays a significant role in ovulation, fertilization, and preimplantation embryonic development.

Original language	English
Pages (from-to)	627-633
Journal	Fertility and Sterility
Volume	64
Issue number	3
Publication status	Published - 1995

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@article{9bcd467eec234052b01987cd4db3af1e,

title = "RU486 inhibits ovulation, fertilization and early embryonic development in rabbits: in vivo and in vitro studies",

abstract = "Objective: To clarify the role of P in ovulation, fertilization, and early embryonic development using RU486, a potent P receptor blocker.Design: Ovulatory efficiency, IVF, and early embryonic development were evaluated after RU486 administration in vivo and in vitro.Setting: Research laboratory of a university hospital.Participants: Mature male and female New Zealand white rabbits.Interventions: Animals were treated with RU486 or vehicle for 3 days before hCG-induced ovulation. Ovaries treated with hCG to induce ovulation were perfused for 6 hours in vitro with RU486 or vehicle. In vitro fertilization was performed in the presence or absence of RU486.Main Outcome Measures: The percentage of mature follicles ovulating (ovulatory efficiency) was determined after in vivo and in vitro treatment. Fertilization, morula, and blastocyst development were evaluated every 24 hours for 120 hours.Results: RU486 significantly inhibited ovulation, fertilization, and early embryonic development.Conclusions: Progesterone plays a significant role in ovulation, fertilization, and preimplantation embryonic development.",

author = "S.H. Chen and Arunasalam Dharmarajan and E.E. Wallach and C. Mastroyannis",

year = "1995",

language = "English",

volume = "64",

pages = "627--633",

journal = "Ferility and Sterility",

issn = "0015-0282",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - RU486 inhibits ovulation, fertilization and early embryonic development in rabbits: in vivo and in vitro studies

AU - Chen, S.H.

AU - Dharmarajan, Arunasalam

AU - Wallach, E.E.

AU - Mastroyannis, C.

PY - 1995

Y1 - 1995

N2 - Objective: To clarify the role of P in ovulation, fertilization, and early embryonic development using RU486, a potent P receptor blocker.Design: Ovulatory efficiency, IVF, and early embryonic development were evaluated after RU486 administration in vivo and in vitro.Setting: Research laboratory of a university hospital.Participants: Mature male and female New Zealand white rabbits.Interventions: Animals were treated with RU486 or vehicle for 3 days before hCG-induced ovulation. Ovaries treated with hCG to induce ovulation were perfused for 6 hours in vitro with RU486 or vehicle. In vitro fertilization was performed in the presence or absence of RU486.Main Outcome Measures: The percentage of mature follicles ovulating (ovulatory efficiency) was determined after in vivo and in vitro treatment. Fertilization, morula, and blastocyst development were evaluated every 24 hours for 120 hours.Results: RU486 significantly inhibited ovulation, fertilization, and early embryonic development.Conclusions: Progesterone plays a significant role in ovulation, fertilization, and preimplantation embryonic development.

AB - Objective: To clarify the role of P in ovulation, fertilization, and early embryonic development using RU486, a potent P receptor blocker.Design: Ovulatory efficiency, IVF, and early embryonic development were evaluated after RU486 administration in vivo and in vitro.Setting: Research laboratory of a university hospital.Participants: Mature male and female New Zealand white rabbits.Interventions: Animals were treated with RU486 or vehicle for 3 days before hCG-induced ovulation. Ovaries treated with hCG to induce ovulation were perfused for 6 hours in vitro with RU486 or vehicle. In vitro fertilization was performed in the presence or absence of RU486.Main Outcome Measures: The percentage of mature follicles ovulating (ovulatory efficiency) was determined after in vivo and in vitro treatment. Fertilization, morula, and blastocyst development were evaluated every 24 hours for 120 hours.Results: RU486 significantly inhibited ovulation, fertilization, and early embryonic development.Conclusions: Progesterone plays a significant role in ovulation, fertilization, and preimplantation embryonic development.

M3 - Article

VL - 64

SP - 627

EP - 633

JO - Ferility and Sterility

JF - Ferility and Sterility

SN - 0015-0282

IS - 3

ER -