Root development impacts on the distribution of phosphatase activity: Improvements in quantification using soil zymography

C. D. Giles; L. Dupuy; G. Boitt; L. K. Brown; L. M. Condron; T. Darch; M. S. A. Blackwell; D. Menezes-Blackburn; C. A. Shand; M. I. Stutter; D. G. Lumsdon; R. Wendler; P. Cooper; C. Wearing; H. Zhang; P. M. Haygarth; T. S. George

doi:10.1016/j.soilbio.2017.08.011

Root development impacts on the distribution of phosphatase activity: Improvements in quantification using soil zymography

C. D. Giles, L. Dupuy, G. Boitt, L. K. Brown, L. M. Condron, T. Darch, M. S. A. Blackwell, D. Menezes-Blackburn, C. A. Shand, M. I. Stutter, D. G. Lumsdon, R. Wendler, P. Cooper, C. Wearing, H. Zhang, P. M. Haygarth, T. S. George

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Zymographic methods for the 2D distribution of phosphatase activity in soils have markedly advanced our understanding of root-soil-microbiota interactions. Robust quantitative approaches for 2D assays, which use 4-methylubelliferyl phosphate (4-MUP), are needed to advance a mechanistic understanding of enzyme behaviour and distribution in soils. We present improvements to the method for phosphatase zymography in rhizobox studies, involving (1) a systematic evaluation of 4-methylumbelliferone (4-MU) based calibration functions in relation to image exposure time and (2) the development of advanced image analysis tools for lateral and longitudinal distributions of phosphatase activity along barley roots (Hordeum vulgare L., cv Optic). Exposure time (

Original language	English
Pages (from-to)	158-166
Number of pages	9
Journal	Soil Biology & Biochemistry
Volume	116
DOIs	https://doi.org/10.1016/j.soilbio.2017.08.011
Publication status	Published - Jan 2018
Externally published	Yes

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Giles, C. D., Dupuy, L., Boitt, G., Brown, L. K., Condron, L. M., Darch, T., Blackwell, M. S. A., Menezes-Blackburn, D., Shand, C. A., Stutter, M. I., Lumsdon, D. G., Wendler, R., Cooper, P., Wearing, C., Zhang, H., Haygarth, P. M., & George, T. S. (2018). Root development impacts on the distribution of phosphatase activity: Improvements in quantification using soil zymography. Soil Biology & Biochemistry, 116, 158-166. https://doi.org/10.1016/j.soilbio.2017.08.011

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title = "Root development impacts on the distribution of phosphatase activity: Improvements in quantification using soil zymography",

abstract = "Zymographic methods for the 2D distribution of phosphatase activity in soils have markedly advanced our understanding of root-soil-microbiota interactions. Robust quantitative approaches for 2D assays, which use 4-methylubelliferyl phosphate (4-MUP), are needed to advance a mechanistic understanding of enzyme behaviour and distribution in soils. We present improvements to the method for phosphatase zymography in rhizobox studies, involving (1) a systematic evaluation of 4-methylumbelliferone (4-MU) based calibration functions in relation to image exposure time and (2) the development of advanced image analysis tools for lateral and longitudinal distributions of phosphatase activity along barley roots (Hordeum vulgare L., cv Optic). Exposure time (",

keywords = "Barley, Image analysis, 4-Methylumbelliferone, Phosphatase, Rhizosphere, Soil zymography, DEMONSTRATING CHEMICAL-CHANGES, NONDESTRUCTIVE METHODS, ENZYME-ACTIVITY, RHIZOSPHERE, 4-METHYLUMBELLIFERONE, FLUORESCENCE, EXUDATION, HOTSPOTS",

author = "Giles, {C. D.} and L. Dupuy and G. Boitt and Brown, {L. K.} and Condron, {L. M.} and T. Darch and Blackwell, {M. S. A.} and D. Menezes-Blackburn and Shand, {C. A.} and Stutter, {M. I.} and Lumsdon, {D. G.} and R. Wendler and P. Cooper and C. Wearing and H. Zhang and Haygarth, {P. M.} and George, {T. S.}",

year = "2018",

month = jan,

doi = "10.1016/j.soilbio.2017.08.011",

language = "English",

volume = "116",

pages = "158--166",

journal = "Soil Biology & Biochemistry",

issn = "0038-0717",

publisher = "Pergamon",

}

Giles, CD, Dupuy, L, Boitt, G, Brown, LK, Condron, LM, Darch, T, Blackwell, MSA, Menezes-Blackburn, D, Shand, CA, Stutter, MI, Lumsdon, DG, Wendler, R, Cooper, P, Wearing, C, Zhang, H, Haygarth, PM & George, TS 2018, 'Root development impacts on the distribution of phosphatase activity: Improvements in quantification using soil zymography', Soil Biology & Biochemistry, vol. 116, pp. 158-166. https://doi.org/10.1016/j.soilbio.2017.08.011

TY - JOUR

T1 - Root development impacts on the distribution of phosphatase activity

T2 - Improvements in quantification using soil zymography

AU - Giles, C. D.

AU - Dupuy, L.

AU - Boitt, G.

AU - Brown, L. K.

AU - Condron, L. M.

AU - Darch, T.

AU - Blackwell, M. S. A.

AU - Menezes-Blackburn, D.

AU - Shand, C. A.

AU - Stutter, M. I.

AU - Lumsdon, D. G.

AU - Wendler, R.

AU - Cooper, P.

AU - Wearing, C.

AU - Zhang, H.

AU - Haygarth, P. M.

AU - George, T. S.

PY - 2018/1

Y1 - 2018/1

N2 - Zymographic methods for the 2D distribution of phosphatase activity in soils have markedly advanced our understanding of root-soil-microbiota interactions. Robust quantitative approaches for 2D assays, which use 4-methylubelliferyl phosphate (4-MUP), are needed to advance a mechanistic understanding of enzyme behaviour and distribution in soils. We present improvements to the method for phosphatase zymography in rhizobox studies, involving (1) a systematic evaluation of 4-methylumbelliferone (4-MU) based calibration functions in relation to image exposure time and (2) the development of advanced image analysis tools for lateral and longitudinal distributions of phosphatase activity along barley roots (Hordeum vulgare L., cv Optic). Exposure time (

AB - Zymographic methods for the 2D distribution of phosphatase activity in soils have markedly advanced our understanding of root-soil-microbiota interactions. Robust quantitative approaches for 2D assays, which use 4-methylubelliferyl phosphate (4-MUP), are needed to advance a mechanistic understanding of enzyme behaviour and distribution in soils. We present improvements to the method for phosphatase zymography in rhizobox studies, involving (1) a systematic evaluation of 4-methylumbelliferone (4-MU) based calibration functions in relation to image exposure time and (2) the development of advanced image analysis tools for lateral and longitudinal distributions of phosphatase activity along barley roots (Hordeum vulgare L., cv Optic). Exposure time (

KW - Barley

KW - Image analysis

KW - 4-Methylumbelliferone

KW - Phosphatase

KW - Rhizosphere

KW - Soil zymography

KW - DEMONSTRATING CHEMICAL-CHANGES

KW - NONDESTRUCTIVE METHODS

KW - ENZYME-ACTIVITY

KW - RHIZOSPHERE

KW - 4-METHYLUMBELLIFERONE

KW - FLUORESCENCE

KW - EXUDATION

KW - HOTSPOTS

U2 - 10.1016/j.soilbio.2017.08.011

DO - 10.1016/j.soilbio.2017.08.011

M3 - Article

SN - 0038-0717

VL - 116

SP - 158

EP - 166

JO - Soil Biology & Biochemistry

JF - Soil Biology & Biochemistry

ER -

Root development impacts on the distribution of phosphatase activity: Improvements in quantification using soil zymography

Abstract

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