TY - JOUR
T1 - Role of sugars and organic acids in regulating the concentration and activity of the alternative oxidase in Poa annua roots
AU - Millenaar, F.F.
AU - Gonzalez-Meler, M.A.
AU - Siedow, J.N.
AU - Wagner, A.K.
AU - Lambers, Hans
PY - 2002
Y1 - 2002
N2 - Detached roots of Poa annua were used to study alternative oxidase protein expression upon the addition of sucrose, glucose, fructose, inositol, mannitol, citrate or malate, at a concentration of 1 or 10 mM for 24 h. After 24 h the capacity of cytochrome c oxidase was decreased equally in all treatments. Only citrate induced the expression of the alternative oxidase, especially at a concentration of 1 mM (15-fold). The activity of the alternative pathway (measured with the O-18-fractionation technique) was not affected by the addition of sucrose for 24 h as compared with time zero. However, after the addition of citrate or mannitol the activity of the alternative pathway decreased to almost zero. The discrepancy between the large increase in alternative oxidase protein concentration when citrate was applied and the concomitant decrease in alternative pathway activity is discussed.
AB - Detached roots of Poa annua were used to study alternative oxidase protein expression upon the addition of sucrose, glucose, fructose, inositol, mannitol, citrate or malate, at a concentration of 1 or 10 mM for 24 h. After 24 h the capacity of cytochrome c oxidase was decreased equally in all treatments. Only citrate induced the expression of the alternative oxidase, especially at a concentration of 1 mM (15-fold). The activity of the alternative pathway (measured with the O-18-fractionation technique) was not affected by the addition of sucrose for 24 h as compared with time zero. However, after the addition of citrate or mannitol the activity of the alternative pathway decreased to almost zero. The discrepancy between the large increase in alternative oxidase protein concentration when citrate was applied and the concomitant decrease in alternative pathway activity is discussed.
U2 - 10.1093/jexbot/53.371.1081
DO - 10.1093/jexbot/53.371.1081
M3 - Article
SN - 0022-0957
VL - 53
SP - 1081
EP - 1088
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 371
ER -