ROLE OF O-2 IN REGULATING TISSUE RESPIRATION IN DOG MUSCLE WORKING INSITU

MC HOGAN, Peter Arthur, DE BEBOUT, PW Hochachka, PD WAGNER

Research output: Contribution to journalArticle

Abstract

This study was designed to investigate the role of tissue oxygenation in some of the factors that are thought to regulate muscle respiration and metabolism. Tissue oxygenation was altered by reductions in O2 delivery (muscle blood flow x arterial O2 content), induced by decreases in arterial PO2 (Pa(O2)). O2 uptake (VO2) was measured in isolated in situ canine gastrocnemius at rest and while working at two stimulation intensities (isometric tetanic contractions at 0.5 and 1 contractions/s) on three separate occasions, with only the level of Pa(O2) (78, 30, and 21 Torr) being different for each occasion. Muscle blood flow was held constant (pump perfusion) at each work intensity for the three different levels of Pa(O2). Muscle biopsies were obtained at the end of each rest and work period. Muscle VO2 was significantly less (P <0.05) at both stimulation intensities for the hypoxemic conditions, whereas [ATP] was reduced only during the highest work intensity during both hypoxemic conditions (31 % reduction at 21 Torr Pa(O2) and 17% at 30 Torr). For each level of Pa(O2), the relationships between the changes that occurred in VO2 and levels of phosphocreatine, ADP, and ATP/ADP . P(i) as the stimulation intensity was increased were significantly correlated; however, the slopes and intercepts of these lines were significantly different for each Pa(O2). Thus a greater change in any of the proposed regulators of tissue respiration (e.g., phosphocreatine, ADP) was required to achieve a given VO2 as Pa(O2) was decreased. These results indicate that VO2 can be dissociated from these proposed regulators of tissue respiration and that this is likely due to an interaction with tissue or mitochondrial O2 tension, thereby demonstrating the importance of O2 as a modulator of the regulators of tissue respiration.

Original languageEnglish
Pages (from-to)728-736
Number of pages9
JournalJournal of Applied Physiology
Volume73
Issue number2
Publication statusPublished - Aug 1992

Cite this

HOGAN, MC., Arthur, P., BEBOUT, DE., Hochachka, PW., & WAGNER, PD. (1992). ROLE OF O-2 IN REGULATING TISSUE RESPIRATION IN DOG MUSCLE WORKING INSITU. Journal of Applied Physiology, 73(2), 728-736.
HOGAN, MC ; Arthur, Peter ; BEBOUT, DE ; Hochachka, PW ; WAGNER, PD. / ROLE OF O-2 IN REGULATING TISSUE RESPIRATION IN DOG MUSCLE WORKING INSITU. In: Journal of Applied Physiology. 1992 ; Vol. 73, No. 2. pp. 728-736.
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abstract = "This study was designed to investigate the role of tissue oxygenation in some of the factors that are thought to regulate muscle respiration and metabolism. Tissue oxygenation was altered by reductions in O2 delivery (muscle blood flow x arterial O2 content), induced by decreases in arterial PO2 (Pa(O2)). O2 uptake (VO2) was measured in isolated in situ canine gastrocnemius at rest and while working at two stimulation intensities (isometric tetanic contractions at 0.5 and 1 contractions/s) on three separate occasions, with only the level of Pa(O2) (78, 30, and 21 Torr) being different for each occasion. Muscle blood flow was held constant (pump perfusion) at each work intensity for the three different levels of Pa(O2). Muscle biopsies were obtained at the end of each rest and work period. Muscle VO2 was significantly less (P <0.05) at both stimulation intensities for the hypoxemic conditions, whereas [ATP] was reduced only during the highest work intensity during both hypoxemic conditions (31 {\%} reduction at 21 Torr Pa(O2) and 17{\%} at 30 Torr). For each level of Pa(O2), the relationships between the changes that occurred in VO2 and levels of phosphocreatine, ADP, and ATP/ADP . P(i) as the stimulation intensity was increased were significantly correlated; however, the slopes and intercepts of these lines were significantly different for each Pa(O2). Thus a greater change in any of the proposed regulators of tissue respiration (e.g., phosphocreatine, ADP) was required to achieve a given VO2 as Pa(O2) was decreased. These results indicate that VO2 can be dissociated from these proposed regulators of tissue respiration and that this is likely due to an interaction with tissue or mitochondrial O2 tension, thereby demonstrating the importance of O2 as a modulator of the regulators of tissue respiration.",
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HOGAN, MC, Arthur, P, BEBOUT, DE, Hochachka, PW & WAGNER, PD 1992, 'ROLE OF O-2 IN REGULATING TISSUE RESPIRATION IN DOG MUSCLE WORKING INSITU' Journal of Applied Physiology, vol. 73, no. 2, pp. 728-736.

ROLE OF O-2 IN REGULATING TISSUE RESPIRATION IN DOG MUSCLE WORKING INSITU. / HOGAN, MC; Arthur, Peter; BEBOUT, DE; Hochachka, PW; WAGNER, PD.

In: Journal of Applied Physiology, Vol. 73, No. 2, 08.1992, p. 728-736.

Research output: Contribution to journalArticle

TY - JOUR

T1 - ROLE OF O-2 IN REGULATING TISSUE RESPIRATION IN DOG MUSCLE WORKING INSITU

AU - HOGAN, MC

AU - Arthur, Peter

AU - BEBOUT, DE

AU - Hochachka, PW

AU - WAGNER, PD

PY - 1992/8

Y1 - 1992/8

N2 - This study was designed to investigate the role of tissue oxygenation in some of the factors that are thought to regulate muscle respiration and metabolism. Tissue oxygenation was altered by reductions in O2 delivery (muscle blood flow x arterial O2 content), induced by decreases in arterial PO2 (Pa(O2)). O2 uptake (VO2) was measured in isolated in situ canine gastrocnemius at rest and while working at two stimulation intensities (isometric tetanic contractions at 0.5 and 1 contractions/s) on three separate occasions, with only the level of Pa(O2) (78, 30, and 21 Torr) being different for each occasion. Muscle blood flow was held constant (pump perfusion) at each work intensity for the three different levels of Pa(O2). Muscle biopsies were obtained at the end of each rest and work period. Muscle VO2 was significantly less (P <0.05) at both stimulation intensities for the hypoxemic conditions, whereas [ATP] was reduced only during the highest work intensity during both hypoxemic conditions (31 % reduction at 21 Torr Pa(O2) and 17% at 30 Torr). For each level of Pa(O2), the relationships between the changes that occurred in VO2 and levels of phosphocreatine, ADP, and ATP/ADP . P(i) as the stimulation intensity was increased were significantly correlated; however, the slopes and intercepts of these lines were significantly different for each Pa(O2). Thus a greater change in any of the proposed regulators of tissue respiration (e.g., phosphocreatine, ADP) was required to achieve a given VO2 as Pa(O2) was decreased. These results indicate that VO2 can be dissociated from these proposed regulators of tissue respiration and that this is likely due to an interaction with tissue or mitochondrial O2 tension, thereby demonstrating the importance of O2 as a modulator of the regulators of tissue respiration.

AB - This study was designed to investigate the role of tissue oxygenation in some of the factors that are thought to regulate muscle respiration and metabolism. Tissue oxygenation was altered by reductions in O2 delivery (muscle blood flow x arterial O2 content), induced by decreases in arterial PO2 (Pa(O2)). O2 uptake (VO2) was measured in isolated in situ canine gastrocnemius at rest and while working at two stimulation intensities (isometric tetanic contractions at 0.5 and 1 contractions/s) on three separate occasions, with only the level of Pa(O2) (78, 30, and 21 Torr) being different for each occasion. Muscle blood flow was held constant (pump perfusion) at each work intensity for the three different levels of Pa(O2). Muscle biopsies were obtained at the end of each rest and work period. Muscle VO2 was significantly less (P <0.05) at both stimulation intensities for the hypoxemic conditions, whereas [ATP] was reduced only during the highest work intensity during both hypoxemic conditions (31 % reduction at 21 Torr Pa(O2) and 17% at 30 Torr). For each level of Pa(O2), the relationships between the changes that occurred in VO2 and levels of phosphocreatine, ADP, and ATP/ADP . P(i) as the stimulation intensity was increased were significantly correlated; however, the slopes and intercepts of these lines were significantly different for each Pa(O2). Thus a greater change in any of the proposed regulators of tissue respiration (e.g., phosphocreatine, ADP) was required to achieve a given VO2 as Pa(O2) was decreased. These results indicate that VO2 can be dissociated from these proposed regulators of tissue respiration and that this is likely due to an interaction with tissue or mitochondrial O2 tension, thereby demonstrating the importance of O2 as a modulator of the regulators of tissue respiration.

KW - FATIGUE

KW - SKELETAL MUSCLE

KW - GAS EXCHANGE

KW - EXERCISE

KW - ACID-BASE BALANCE

KW - LACTATE

KW - LACTIC ACID

KW - MITOCHONDRIAL RESPIRATION

KW - PHOSPHOCREATINE

KW - RED MUSCLE

KW - SKELETAL-MUSCLE

KW - INTRACELLULAR PO2

KW - OXYGEN DEPENDENCE

KW - ENERGY-METABOLISM

KW - MUSCULAR FATIGUE

KW - HYPOXIA

KW - CONTRACTIONS

KW - CONSUMPTION

M3 - Article

VL - 73

SP - 728

EP - 736

JO - Journal of applied physiology (Bethesda, Md. : 1985)

JF - Journal of applied physiology (Bethesda, Md. : 1985)

SN - 1522-1601

IS - 2

ER -