RNF187 is Downregulated Following NF-?B Inhibition in Late Erythroblasts

Luke Forster, Jill Finlayson, Reza Ghassemifar

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    © 2016, Springer Science+Business Media New York.Beta (ß)-thalassaemic erythroblasts grown in vitro have reduced nuclear factor kappa B (NF-?B) pathway gene expression. By inhibiting this pathway in erythroblasts from normal individuals, important downstream genes affected by this inhibition can be identified. Bay 11-7082 is a potent inhibitor of the NF-?B pathway, it acts irreversibly, inhibiting NF-?B activation by blocking tumor necrosis factor alpha (TNF-a)-induced phosphorylation of the inhibitory I?B subunit thereby preventing NF-?B activation. In this study, hematopoietic stem cells were isolated from the peripheral blood of 6 healthy individuals and were then cultured for 14 days in conditions which promote erythroid differentiation. Following erythroid lineage enrichment, these cells were stimulated with TNFa or inhibited with Bay 11-7082. Subsequent RNA isolation and gene expression analyses were performed using pooled cDNA with custom PCR arrays. Genes of interest were examined individually on non-pooled samples. Our data identified RNF187, a RING finger domain gene as being downregulated in response to NF-?B inhibition.
    Original languageEnglish
    Pages (from-to)714-721
    JournalBiochemical Genetics
    Volume54
    Issue number5
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    erythroblasts
    Erythroblasts
    Down-Regulation
    Genes
    Gene expression
    gene expression
    gene
    RING Finger Domains
    Chemical activation
    Gene Expression
    Phosphorylation
    genes
    NF-kappa B
    Hematopoietic Stem Cells
    Stem cells
    tumor
    tumor necrosis factor-alpha
    RNA
    inhibitor
    phosphorylation

    Cite this

    Forster, Luke ; Finlayson, Jill ; Ghassemifar, Reza. / RNF187 is Downregulated Following NF-?B Inhibition in Late Erythroblasts. In: Biochemical Genetics. 2016 ; Vol. 54, No. 5. pp. 714-721.
    @article{15d855099ac74f859bb19627bff9d623,
    title = "RNF187 is Downregulated Following NF-?B Inhibition in Late Erythroblasts",
    abstract = "{\circledC} 2016, Springer Science+Business Media New York.Beta ({\ss})-thalassaemic erythroblasts grown in vitro have reduced nuclear factor kappa B (NF-?B) pathway gene expression. By inhibiting this pathway in erythroblasts from normal individuals, important downstream genes affected by this inhibition can be identified. Bay 11-7082 is a potent inhibitor of the NF-?B pathway, it acts irreversibly, inhibiting NF-?B activation by blocking tumor necrosis factor alpha (TNF-a)-induced phosphorylation of the inhibitory I?B subunit thereby preventing NF-?B activation. In this study, hematopoietic stem cells were isolated from the peripheral blood of 6 healthy individuals and were then cultured for 14 days in conditions which promote erythroid differentiation. Following erythroid lineage enrichment, these cells were stimulated with TNFa or inhibited with Bay 11-7082. Subsequent RNA isolation and gene expression analyses were performed using pooled cDNA with custom PCR arrays. Genes of interest were examined individually on non-pooled samples. Our data identified RNF187, a RING finger domain gene as being downregulated in response to NF-?B inhibition.",
    author = "Luke Forster and Jill Finlayson and Reza Ghassemifar",
    year = "2016",
    doi = "10.1007/s10528-016-9750-0",
    language = "English",
    volume = "54",
    pages = "714--721",
    journal = "Biochemical Genetics",
    issn = "0006-2928",
    publisher = "Springer",
    number = "5",

    }

    RNF187 is Downregulated Following NF-?B Inhibition in Late Erythroblasts. / Forster, Luke; Finlayson, Jill; Ghassemifar, Reza.

    In: Biochemical Genetics, Vol. 54, No. 5, 2016, p. 714-721.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - RNF187 is Downregulated Following NF-?B Inhibition in Late Erythroblasts

    AU - Forster, Luke

    AU - Finlayson, Jill

    AU - Ghassemifar, Reza

    PY - 2016

    Y1 - 2016

    N2 - © 2016, Springer Science+Business Media New York.Beta (ß)-thalassaemic erythroblasts grown in vitro have reduced nuclear factor kappa B (NF-?B) pathway gene expression. By inhibiting this pathway in erythroblasts from normal individuals, important downstream genes affected by this inhibition can be identified. Bay 11-7082 is a potent inhibitor of the NF-?B pathway, it acts irreversibly, inhibiting NF-?B activation by blocking tumor necrosis factor alpha (TNF-a)-induced phosphorylation of the inhibitory I?B subunit thereby preventing NF-?B activation. In this study, hematopoietic stem cells were isolated from the peripheral blood of 6 healthy individuals and were then cultured for 14 days in conditions which promote erythroid differentiation. Following erythroid lineage enrichment, these cells were stimulated with TNFa or inhibited with Bay 11-7082. Subsequent RNA isolation and gene expression analyses were performed using pooled cDNA with custom PCR arrays. Genes of interest were examined individually on non-pooled samples. Our data identified RNF187, a RING finger domain gene as being downregulated in response to NF-?B inhibition.

    AB - © 2016, Springer Science+Business Media New York.Beta (ß)-thalassaemic erythroblasts grown in vitro have reduced nuclear factor kappa B (NF-?B) pathway gene expression. By inhibiting this pathway in erythroblasts from normal individuals, important downstream genes affected by this inhibition can be identified. Bay 11-7082 is a potent inhibitor of the NF-?B pathway, it acts irreversibly, inhibiting NF-?B activation by blocking tumor necrosis factor alpha (TNF-a)-induced phosphorylation of the inhibitory I?B subunit thereby preventing NF-?B activation. In this study, hematopoietic stem cells were isolated from the peripheral blood of 6 healthy individuals and were then cultured for 14 days in conditions which promote erythroid differentiation. Following erythroid lineage enrichment, these cells were stimulated with TNFa or inhibited with Bay 11-7082. Subsequent RNA isolation and gene expression analyses were performed using pooled cDNA with custom PCR arrays. Genes of interest were examined individually on non-pooled samples. Our data identified RNF187, a RING finger domain gene as being downregulated in response to NF-?B inhibition.

    U2 - 10.1007/s10528-016-9750-0

    DO - 10.1007/s10528-016-9750-0

    M3 - Article

    VL - 54

    SP - 714

    EP - 721

    JO - Biochemical Genetics

    JF - Biochemical Genetics

    SN - 0006-2928

    IS - 5

    ER -