RNF187 is Downregulated Following NF-?B Inhibition in Late Erythroblasts

Luke Forster, Jill Finlayson, Reza Ghassemifar

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)


    © 2016, Springer Science+Business Media New York.Beta (ß)-thalassaemic erythroblasts grown in vitro have reduced nuclear factor kappa B (NF-?B) pathway gene expression. By inhibiting this pathway in erythroblasts from normal individuals, important downstream genes affected by this inhibition can be identified. Bay 11-7082 is a potent inhibitor of the NF-?B pathway, it acts irreversibly, inhibiting NF-?B activation by blocking tumor necrosis factor alpha (TNF-a)-induced phosphorylation of the inhibitory I?B subunit thereby preventing NF-?B activation. In this study, hematopoietic stem cells were isolated from the peripheral blood of 6 healthy individuals and were then cultured for 14 days in conditions which promote erythroid differentiation. Following erythroid lineage enrichment, these cells were stimulated with TNFa or inhibited with Bay 11-7082. Subsequent RNA isolation and gene expression analyses were performed using pooled cDNA with custom PCR arrays. Genes of interest were examined individually on non-pooled samples. Our data identified RNF187, a RING finger domain gene as being downregulated in response to NF-?B inhibition.
    Original languageEnglish
    Pages (from-to)714-721
    Number of pages8
    JournalBiochemical Genetics
    Issue number5
    Publication statusPublished - 1 Oct 2016


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