Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance

Yusuke Kurokawa, Keisuke Nagai, Phung Danh Huan, Kousuke Shimazaki, Huangqi Qu, Yoshinao Mori, Yosuke Toda, Takeshi Kuroha, Nagao Hayashi, Saori Aiga, Jun Ichi Itoh, Atsushi Yoshimura, Yuko Sasaki-Sekimoto, Hiroyuki Ohta, Mie Shimojima, Al Imran Malik, Ole Pedersen, Timothy David Colmer, Motoyuki Ashikari

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Floods impede gas (O2 and CO2) exchange between plants and the environment. A mechanism to enhance plant gas exchange under water comprises gas films on hydrophobic leaves, but the genetic regulation of this mechanism is unknown. We used a rice mutant (dripping wet leaf 7, drp7) which does not retain gas films on leaves, and its wild-type (Kinmaze), in gene discovery for this trait. Gene complementation was tested in transgenic lines. Functional properties of leaves as related to gas film retention and underwater photosynthesis were evaluated. Leaf Gas Film 1 (LGF1) was identified as the gene determining leaf gas films. LGF1 regulates C30 primary alcohol synthesis, which is necessary for abundant epicuticular wax platelets, leaf hydrophobicity and gas films on submerged leaves. This trait enhanced underwater photosynthesis 8.2-fold and contributes to submergence tolerance. Gene function was verified by a complementation test of LGF1 expressed in the drp7 mutant background, which restored C30 primary alcohol synthesis, wax platelet abundance, leaf hydrophobicity, gas film retention, and underwater photosynthesis. The discovery of LGF1 provides an opportunity to better understand variation amongst rice genotypes for gas film retention ability and to target various alleles in breeding for improved submergence tolerance for yield stability in flood-prone areas.

Original languageEnglish
Pages (from-to)1558-1569
Number of pages12
JournalNew Phytologist
Volume218
Issue number4
DOIs
Publication statusPublished - 1 Jun 2018

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    Kurokawa, Y., Nagai, K., Huan, P. D., Shimazaki, K., Qu, H., Mori, Y., ... Ashikari, M. (2018). Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance. New Phytologist, 218(4), 1558-1569. https://doi.org/10.1111/nph.15070