Rhoifolin ameliorates titanium particle-stimulated osteolysis and attenuates osteoclastogenesis via RANKL-induced NF-κB and MAPK pathways

Shijie Liao, Fangmin Song, Wenyu Feng, Xiaofei Ding, Jun Yao, Huijie Song, Yun Liu, Shiting Ma, Ziyi Wang, Xixi Lin, Jiake Xu, Jinmin Zhao, Qian Liu

Research output: Contribution to journalArticle

Abstract

Prosthesis loosening is a highly troublesome clinical problem following total joint arthroplasty. Wear-particle-induced osteoclastogenesis has been shown to be the primary cause of periprosthetic osteolysis that eventually leads to aseptic prosthesis loosening. Therefore, inhibiting osteoclastogenesis is a promising strategy to control periprosthetic osteolysis. The possible mechanism of action of rhoifolin on osteoclastogenesis and titanium particle-induced calvarial osteolysis was examined in this study. The in vitro study showed that rhoifolin could strongly suppress the receptor activators of nuclear factor-κB (NF-κB) ligand-stimulated osteoclastogenesis, hydroxyapatite resorption, F-actin formation, and the gene expression of osteoclast-related genes. Western blot analysis illustrated that rhoifolin could attenuate the NF-κB and mitogen-activated protein kinase pathways, and the expression of transcriptional factors nuclear factor of activated T cells 1 (NFATc1) and c-Fos. Further studies indicated that rhoifolin inhibited p65 translocation to the nucleus and the activity of NFATc1 and NF-κB rhoifolin could decrease the number of tartrate-resistant acid phosphate-positive osteoclasts and titanium particle-induced C57 mouse calvarial bone loss in vivo. In conclusion, our results suggest that rhoifolin can ameliorate the osteoclasts-stimulated osteolysis, and may be a potential agent for the treatment of prosthesis loosening.

Original languageEnglish
Pages (from-to)17600-17611
Number of pages12
JournalJournal of Cellular Physiology
Volume234
Issue number10
DOIs
Publication statusPublished - 1 Oct 2019

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Osteolysis
Titanium
Osteogenesis
Prosthesis Failure
Osteoclasts
NFATC Transcription Factors
TCF Transcription Factors
Arthroplasty
Durapatite
Cytoplasmic and Nuclear Receptors
Mitogen-Activated Protein Kinases
Gene expression
rhoifolin
Actins
Bone
Genes
Joints
Western Blotting
Phosphates
Wear of materials

Cite this

Liao, Shijie ; Song, Fangmin ; Feng, Wenyu ; Ding, Xiaofei ; Yao, Jun ; Song, Huijie ; Liu, Yun ; Ma, Shiting ; Wang, Ziyi ; Lin, Xixi ; Xu, Jiake ; Zhao, Jinmin ; Liu, Qian. / Rhoifolin ameliorates titanium particle-stimulated osteolysis and attenuates osteoclastogenesis via RANKL-induced NF-κB and MAPK pathways. In: Journal of Cellular Physiology. 2019 ; Vol. 234, No. 10. pp. 17600-17611.
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title = "Rhoifolin ameliorates titanium particle-stimulated osteolysis and attenuates osteoclastogenesis via RANKL-induced NF-κB and MAPK pathways",
abstract = "Prosthesis loosening is a highly troublesome clinical problem following total joint arthroplasty. Wear-particle-induced osteoclastogenesis has been shown to be the primary cause of periprosthetic osteolysis that eventually leads to aseptic prosthesis loosening. Therefore, inhibiting osteoclastogenesis is a promising strategy to control periprosthetic osteolysis. The possible mechanism of action of rhoifolin on osteoclastogenesis and titanium particle-induced calvarial osteolysis was examined in this study. The in vitro study showed that rhoifolin could strongly suppress the receptor activators of nuclear factor-κB (NF-κB) ligand-stimulated osteoclastogenesis, hydroxyapatite resorption, F-actin formation, and the gene expression of osteoclast-related genes. Western blot analysis illustrated that rhoifolin could attenuate the NF-κB and mitogen-activated protein kinase pathways, and the expression of transcriptional factors nuclear factor of activated T cells 1 (NFATc1) and c-Fos. Further studies indicated that rhoifolin inhibited p65 translocation to the nucleus and the activity of NFATc1 and NF-κB rhoifolin could decrease the number of tartrate-resistant acid phosphate-positive osteoclasts and titanium particle-induced C57 mouse calvarial bone loss in vivo. In conclusion, our results suggest that rhoifolin can ameliorate the osteoclasts-stimulated osteolysis, and may be a potential agent for the treatment of prosthesis loosening.",
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Liao, S, Song, F, Feng, W, Ding, X, Yao, J, Song, H, Liu, Y, Ma, S, Wang, Z, Lin, X, Xu, J, Zhao, J & Liu, Q 2019, 'Rhoifolin ameliorates titanium particle-stimulated osteolysis and attenuates osteoclastogenesis via RANKL-induced NF-κB and MAPK pathways' Journal of Cellular Physiology, vol. 234, no. 10, pp. 17600-17611. https://doi.org/10.1002/jcp.28384

Rhoifolin ameliorates titanium particle-stimulated osteolysis and attenuates osteoclastogenesis via RANKL-induced NF-κB and MAPK pathways. / Liao, Shijie; Song, Fangmin; Feng, Wenyu; Ding, Xiaofei; Yao, Jun; Song, Huijie; Liu, Yun; Ma, Shiting; Wang, Ziyi; Lin, Xixi; Xu, Jiake; Zhao, Jinmin; Liu, Qian.

In: Journal of Cellular Physiology, Vol. 234, No. 10, 01.10.2019, p. 17600-17611.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rhoifolin ameliorates titanium particle-stimulated osteolysis and attenuates osteoclastogenesis via RANKL-induced NF-κB and MAPK pathways

AU - Liao, Shijie

AU - Song, Fangmin

AU - Feng, Wenyu

AU - Ding, Xiaofei

AU - Yao, Jun

AU - Song, Huijie

AU - Liu, Yun

AU - Ma, Shiting

AU - Wang, Ziyi

AU - Lin, Xixi

AU - Xu, Jiake

AU - Zhao, Jinmin

AU - Liu, Qian

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Prosthesis loosening is a highly troublesome clinical problem following total joint arthroplasty. Wear-particle-induced osteoclastogenesis has been shown to be the primary cause of periprosthetic osteolysis that eventually leads to aseptic prosthesis loosening. Therefore, inhibiting osteoclastogenesis is a promising strategy to control periprosthetic osteolysis. The possible mechanism of action of rhoifolin on osteoclastogenesis and titanium particle-induced calvarial osteolysis was examined in this study. The in vitro study showed that rhoifolin could strongly suppress the receptor activators of nuclear factor-κB (NF-κB) ligand-stimulated osteoclastogenesis, hydroxyapatite resorption, F-actin formation, and the gene expression of osteoclast-related genes. Western blot analysis illustrated that rhoifolin could attenuate the NF-κB and mitogen-activated protein kinase pathways, and the expression of transcriptional factors nuclear factor of activated T cells 1 (NFATc1) and c-Fos. Further studies indicated that rhoifolin inhibited p65 translocation to the nucleus and the activity of NFATc1 and NF-κB rhoifolin could decrease the number of tartrate-resistant acid phosphate-positive osteoclasts and titanium particle-induced C57 mouse calvarial bone loss in vivo. In conclusion, our results suggest that rhoifolin can ameliorate the osteoclasts-stimulated osteolysis, and may be a potential agent for the treatment of prosthesis loosening.

AB - Prosthesis loosening is a highly troublesome clinical problem following total joint arthroplasty. Wear-particle-induced osteoclastogenesis has been shown to be the primary cause of periprosthetic osteolysis that eventually leads to aseptic prosthesis loosening. Therefore, inhibiting osteoclastogenesis is a promising strategy to control periprosthetic osteolysis. The possible mechanism of action of rhoifolin on osteoclastogenesis and titanium particle-induced calvarial osteolysis was examined in this study. The in vitro study showed that rhoifolin could strongly suppress the receptor activators of nuclear factor-κB (NF-κB) ligand-stimulated osteoclastogenesis, hydroxyapatite resorption, F-actin formation, and the gene expression of osteoclast-related genes. Western blot analysis illustrated that rhoifolin could attenuate the NF-κB and mitogen-activated protein kinase pathways, and the expression of transcriptional factors nuclear factor of activated T cells 1 (NFATc1) and c-Fos. Further studies indicated that rhoifolin inhibited p65 translocation to the nucleus and the activity of NFATc1 and NF-κB rhoifolin could decrease the number of tartrate-resistant acid phosphate-positive osteoclasts and titanium particle-induced C57 mouse calvarial bone loss in vivo. In conclusion, our results suggest that rhoifolin can ameliorate the osteoclasts-stimulated osteolysis, and may be a potential agent for the treatment of prosthesis loosening.

KW - osteoclast

KW - RANKL

KW - rhoifolin

KW - titanium particle

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