Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique

Alicia A. Annamalay, M. Lanaspa, Siew-Kim Khoo, L. Madrid, S. Acacio, Guicheng Zhang, Ingrid A. Laing, J. Gern, Jack Goldblatt, Joelene Bizzintino, Deborah Lehmann, Peter N. Le Souef, Q. Bassat

Research output: Contribution to journalArticlepeer-review

14 Citations (Web of Science)


objectives To describe the prevalence of human rhinovirus (RV) species in children hospitalised
with pneumonia in Manhica, Mozambique, and the associations between RV species and
demographic, clinical and laboratory features.
methods Nasopharyngeal aspirates were collected from children 0 to 10 years of age (n = 277)
presenting to Manhica District Hospital with clinical pneumonia. Blood samples were collected for
HIV and malaria testing, blood culture and full blood counts, and a chest X-ray was performed. A
panel of common respiratory viruses was investigated using two independent multiplex RT-PCR
assays with primers specific for each virus and viral type. RV species and genotypes were identified
by seminested PCR assays, sequencing and phylogenetic tree analyses.
results At least one respiratory virus was identified in 206 (74.4%) children hospitalised with
clinical pneumonia. RV was the most common virus identified in both HIV-infected (17 of 38,
44.7%) and HIV-uninfected (74 of 237, 31.2%; P = 0.100) children. RV-A was the most common
RV species identified (47 of 275, 17.0%), followed by RV-C (35/275, 12.6%) and RV-B (8/275,
2.9%). Clinical presentation of the different RV species was similar and overlapping, with no
particular species being associated with specific clinical features.
conclusions RV-A and RV-C were the most common respiratory viruses identified in children
hospitalised with clinical pneumonia in Manhica. Clinical presentation of RV-A and RV-C was
similar and overlapping
Original languageEnglish
Pages (from-to)1171-1180
Number of pages10
Issue number9
Early online date26 Jul 2016
Publication statusPublished - 1 Sept 2016


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