Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique

Alicia A. Annamalay; M. Lanaspa; Siew-Kim Khoo; L. Madrid; S. Acacio; Guicheng Zhang; Ingrid A. Laing; J. Gern; Jack Goldblatt; Joelene Bizzintino; Deborah Lehmann; Peter N. Le Souef; Q. Bassat

doi:10.1111/tmi.12743

Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique

Alicia A. Annamalay, M. Lanaspa, Siew-Kim Khoo, L. Madrid, S. Acacio, Guicheng Zhang, Ingrid A. Laing, J. Gern, Jack Goldblatt, Joelene Bizzintino, Deborah Lehmann, Peter N. Le Souef, Q. Bassat

UWA Centre for Child Health Research (affiliated with the The Kids Research Institute Australia)

Research output: Contribution to journal › Article › peer-review

15 Citations (Web of Science)

Abstract

objectives To describe the prevalence of human rhinovirus (RV) species in children hospitalised
with pneumonia in Manhica, Mozambique, and the associations between RV species and
demographic, clinical and laboratory features.
methods Nasopharyngeal aspirates were collected from children 0 to 10 years of age (n = 277)
presenting to Manhica District Hospital with clinical pneumonia. Blood samples were collected for
HIV and malaria testing, blood culture and full blood counts, and a chest X-ray was performed. A
panel of common respiratory viruses was investigated using two independent multiplex RT-PCR
assays with primers speciﬁc for each virus and viral type. RV species and genotypes were identiﬁed
by seminested PCR assays, sequencing and phylogenetic tree analyses.
results At least one respiratory virus was identiﬁed in 206 (74.4%) children hospitalised with
clinical pneumonia. RV was the most common virus identiﬁed in both HIV-infected (17 of 38,
44.7%) and HIV-uninfected (74 of 237, 31.2%; P = 0.100) children. RV-A was the most common
RV species identiﬁed (47 of 275, 17.0%), followed by RV-C (35/275, 12.6%) and RV-B (8/275,
2.9%). Clinical presentation of the different RV species was similar and overlapping, with no
particular species being associated with speciﬁc clinical features.
conclusions RV-A and RV-C were the most common respiratory viruses identiﬁed in children
hospitalised with clinical pneumonia in Manhica. Clinical presentation of RV-A and RV-C was
similar and overlapping

Original language	English
Pages (from-to)	1171-1180
Number of pages	10
Journal	TROPICAL MEDICINE & INTERNATIONAL HEALTH
Volume	21
Issue number	9
Early online date	26 Jul 2016
DOIs	https://doi.org/10.1111/tmi.12743
Publication status	Published - 1 Sept 2016

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10.1111/tmi.12743

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Annamalay, A. A., Lanaspa, M., Khoo, S.-K., Madrid, L., Acacio, S., Zhang, G., Laing, I. A., Gern, J., Goldblatt, J., Bizzintino, J., Lehmann, D., Le Souef, P. N., & Bassat, Q. (2016). Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 21(9), 1171-1180. https://doi.org/10.1111/tmi.12743

@article{4c7431fda0904cf7a27f4c561cf7afab,

title = "Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique",

abstract = "objectives To describe the prevalence of human rhinovirus (RV) species in children hospitalisedwith pneumonia in Manhica, Mozambique, and the associations between RV species anddemographic, clinical and laboratory features.methods Nasopharyngeal aspirates were collected from children 0 to 10 years of age (n = 277)presenting to Manhica District Hospital with clinical pneumonia. Blood samples were collected forHIV and malaria testing, blood culture and full blood counts, and a chest X-ray was performed. Apanel of common respiratory viruses was investigated using two independent multiplex RT-PCRassays with primers speciﬁc for each virus and viral type. RV species and genotypes were identiﬁedby seminested PCR assays, sequencing and phylogenetic tree analyses.results At least one respiratory virus was identiﬁed in 206 (74.4%) children hospitalised withclinical pneumonia. RV was the most common virus identiﬁed in both HIV-infected (17 of 38,44.7%) and HIV-uninfected (74 of 237, 31.2%; P = 0.100) children. RV-A was the most commonRV species identiﬁed (47 of 275, 17.0%), followed by RV-C (35/275, 12.6%) and RV-B (8/275,2.9%). Clinical presentation of the different RV species was similar and overlapping, with noparticular species being associated with speciﬁc clinical features.conclusions RV-A and RV-C were the most common respiratory viruses identiﬁed in childrenhospitalised with clinical pneumonia in Manhica. Clinical presentation of RV-A and RV-C wassimilar and overlapping",

author = "Annamalay, {Alicia A.} and M. Lanaspa and Siew-Kim Khoo and L. Madrid and S. Acacio and Guicheng Zhang and Laing, {Ingrid A.} and J. Gern and Jack Goldblatt and Joelene Bizzintino and Deborah Lehmann and {Le Souef}, {Peter N.} and Q. Bassat",

year = "2016",

month = sep,

day = "1",

doi = "10.1111/tmi.12743",

language = "English",

volume = "21",

pages = "1171--1180",

journal = "TROPICAL MEDICINE & INTERNATIONAL HEALTH",

issn = "1360-2276",

publisher = "John Wiley & Sons",

number = "9",

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TY - JOUR

T1 - Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique

AU - Annamalay, Alicia A.

AU - Lanaspa, M.

AU - Khoo, Siew-Kim

AU - Madrid, L.

AU - Acacio, S.

AU - Zhang, Guicheng

AU - Laing, Ingrid A.

AU - Gern, J.

AU - Goldblatt, Jack

AU - Bizzintino, Joelene

AU - Lehmann, Deborah

AU - Le Souef, Peter N.

AU - Bassat, Q.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - objectives To describe the prevalence of human rhinovirus (RV) species in children hospitalisedwith pneumonia in Manhica, Mozambique, and the associations between RV species anddemographic, clinical and laboratory features.methods Nasopharyngeal aspirates were collected from children 0 to 10 years of age (n = 277)presenting to Manhica District Hospital with clinical pneumonia. Blood samples were collected forHIV and malaria testing, blood culture and full blood counts, and a chest X-ray was performed. Apanel of common respiratory viruses was investigated using two independent multiplex RT-PCRassays with primers speciﬁc for each virus and viral type. RV species and genotypes were identiﬁedby seminested PCR assays, sequencing and phylogenetic tree analyses.results At least one respiratory virus was identiﬁed in 206 (74.4%) children hospitalised withclinical pneumonia. RV was the most common virus identiﬁed in both HIV-infected (17 of 38,44.7%) and HIV-uninfected (74 of 237, 31.2%; P = 0.100) children. RV-A was the most commonRV species identiﬁed (47 of 275, 17.0%), followed by RV-C (35/275, 12.6%) and RV-B (8/275,2.9%). Clinical presentation of the different RV species was similar and overlapping, with noparticular species being associated with speciﬁc clinical features.conclusions RV-A and RV-C were the most common respiratory viruses identiﬁed in childrenhospitalised with clinical pneumonia in Manhica. Clinical presentation of RV-A and RV-C wassimilar and overlapping

AB - objectives To describe the prevalence of human rhinovirus (RV) species in children hospitalisedwith pneumonia in Manhica, Mozambique, and the associations between RV species anddemographic, clinical and laboratory features.methods Nasopharyngeal aspirates were collected from children 0 to 10 years of age (n = 277)presenting to Manhica District Hospital with clinical pneumonia. Blood samples were collected forHIV and malaria testing, blood culture and full blood counts, and a chest X-ray was performed. Apanel of common respiratory viruses was investigated using two independent multiplex RT-PCRassays with primers speciﬁc for each virus and viral type. RV species and genotypes were identiﬁedby seminested PCR assays, sequencing and phylogenetic tree analyses.results At least one respiratory virus was identiﬁed in 206 (74.4%) children hospitalised withclinical pneumonia. RV was the most common virus identiﬁed in both HIV-infected (17 of 38,44.7%) and HIV-uninfected (74 of 237, 31.2%; P = 0.100) children. RV-A was the most commonRV species identiﬁed (47 of 275, 17.0%), followed by RV-C (35/275, 12.6%) and RV-B (8/275,2.9%). Clinical presentation of the different RV species was similar and overlapping, with noparticular species being associated with speciﬁc clinical features.conclusions RV-A and RV-C were the most common respiratory viruses identiﬁed in childrenhospitalised with clinical pneumonia in Manhica. Clinical presentation of RV-A and RV-C wassimilar and overlapping

U2 - 10.1111/tmi.12743

DO - 10.1111/tmi.12743

M3 - Article

C2 - 27353724

SN - 1360-2276

VL - 21

SP - 1171

EP - 1180

JO - TROPICAL MEDICINE & INTERNATIONAL HEALTH

JF - TROPICAL MEDICINE & INTERNATIONAL HEALTH

IS - 9

ER -

Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique

Abstract

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