TY - JOUR
T1 - Revealing the transfer pathways of cyanobacterial-fixed N into the boreal forest through the feather-moss microbiome
AU - Arróniz-Crespo, María
AU - Bougoure, Jeremy
AU - Murphy, Daniel V.
AU - Cutler, Nick A.
AU - Souza-Egipsy, Virginia
AU - Chaput, Dominique L.
AU - Jones, Davey L.
AU - Ostle, Nicholas
AU - Wade, Stephen C.
AU - Clode, Peta L.
AU - DeLuca, Thomas H.
N1 - Funding Information:
We thank Dr Karen Moore, Audrey Farbos and Dr Anja Nenninger for help during molecular analysis. We are grateful to Sarah Chesworth and Gwen Lancashire (Bangor University, UK) and Greger Hörnberg and Ingela Bergman from Silvermuseet (Arjeplog, Sweden) providing assistance with field and lab work. We thank Silvia Pressel and Jeffrey G. Duckett (Natural History Museum, London) for their useful advice on resin embedding protocol for Pleurozium schreberi shoots. Simon Oakley, Kelly Mason (UK CEH Lancaster, UK) and Lorna E. Street (Heriot-Watt University, UK) for their useful comments during workshops and group meetings. We acknowledge support from Swedish Research Council grant FORMAS 2003-618 (to TD); NERC grant NE/I027150/1 (to TD and DJ); Australian Research Council Future Fellowship FT110100246 (to DM). The authors acknowledge the use of the Australian Microscopy and Microanalysis Research Facility (CMCA, The University of Western Australia); the Advanced Microscopy and Bioimaging Facility (Aberystwyth University, UK); the Servicio de Microscopı́a Electrónica at the Instituto Ciencias Agrarias (CSIC, Spain); the Exeter Sequencing Service facility, supported by the Wellcome Trust Institutional Strategic Support Fund (WT097835MF), the Wellcome Trust Multi User Equipment Award (WT101650MA), Medical Research Council Clinical Infrastructure Funding (MR/M008924/1) and the BBSRC LOLA award (BB/K003240/1).
Funding Information:
MA-C was also supported by the Comunidad de Madrid (Spain) and European Structural and Investment Funds (projects AGRISOST-CM S2013/ABI2717 and S2018/BAA4330). Acknowledgments
Publisher Copyright:
Copyright © 2022 Arróniz-Crespo, Bougoure, Murphy, Cutler, Souza-Egipsy, Chaput, Jones, Ostle, Wade, Clode and DeLuca.
PY - 2022/12/9
Y1 - 2022/12/9
N2 - Introduction: Biological N2 fixation in feather-mosses is one of the largest inputs of new nitrogen (N) to boreal forest ecosystems; however, revealing the fate of newly fixed N within the bryosphere (i.e. bryophytes and their associated organisms) remains uncertain. Methods: Herein, we combined 15N tracers, high resolution secondary ion mass-spectrometry (NanoSIMS) and a molecular survey of bacterial, fungal and diazotrophic communities, to determine the origin and transfer pathways of newly fixed N2 within feather-moss (Pleurozium schreberi) and its associated microbiome. Results: NanoSIMS images reveal that newly fixed N2, derived from cyanobacteria, is incorporated into moss tissues and associated bacteria, fungi and micro-algae. Discussion: These images demonstrate that previous assumptions that newly fixed N2 is sequestered into moss tissue and only released by decomposition are not correct. We provide the first empirical evidence of new pathways for N2 fixed in feather-mosses to enter the boreal forest ecosystem (i.e. through its microbiome) and discuss the implications for wider ecosystem function.
AB - Introduction: Biological N2 fixation in feather-mosses is one of the largest inputs of new nitrogen (N) to boreal forest ecosystems; however, revealing the fate of newly fixed N within the bryosphere (i.e. bryophytes and their associated organisms) remains uncertain. Methods: Herein, we combined 15N tracers, high resolution secondary ion mass-spectrometry (NanoSIMS) and a molecular survey of bacterial, fungal and diazotrophic communities, to determine the origin and transfer pathways of newly fixed N2 within feather-moss (Pleurozium schreberi) and its associated microbiome. Results: NanoSIMS images reveal that newly fixed N2, derived from cyanobacteria, is incorporated into moss tissues and associated bacteria, fungi and micro-algae. Discussion: These images demonstrate that previous assumptions that newly fixed N2 is sequestered into moss tissue and only released by decomposition are not correct. We provide the first empirical evidence of new pathways for N2 fixed in feather-mosses to enter the boreal forest ecosystem (i.e. through its microbiome) and discuss the implications for wider ecosystem function.
KW - biological N fixation
KW - boreal forest
KW - moss microbiome
KW - moss-cyanobacteria associations
KW - NanoSIMS
KW - nitrogen cycling
KW - Pleurozium schreberi
UR - http://www.scopus.com/inward/record.url?scp=85144998544&partnerID=8YFLogxK
U2 - 10.3389/fpls.2022.1036258
DO - 10.3389/fpls.2022.1036258
M3 - Article
C2 - 36570951
AN - SCOPUS:85144998544
SN - 1664-462X
VL - 13
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 1036258
ER -