Projects per year
Abstract
© 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd
Mitochondria are crucial for plant viability and are able to communicate information on their functional status to the cellular nucleus via retrograde signalling, thereby affecting gene expression. It is currently unclear if retrograde signalling in response to constitutive mitochondrial biogenesis defects is mediated by the same pathways as those triggered during acute mitochondrial dysfunction. Furthermore, it is unknown if retrograde signalling can effectively improve plant performance when mitochondrial function is constitutively impaired. Here we show that retrograde signalling in mutants defective in mitochondrial proteins RNA polymerase rpotmp or prohibitin atphb3 can be suppressed by knocking out the transcription factor ANAC017. Genome-wide RNA-seq expression analysis revealed that ANAC017 is almost solely responsible for the most dramatic transcriptional changes common to rpotmp and atphb3 mutants, regulating classical marker genes such as alternative oxidase 1a (AOX1a) and also previously-uncharacterised DUF295 genes that appear to be new retrograde markers. In contrast, ANAC017 does not regulate intra-mitochondrial gene expression or transcriptional changes unique to either rpotmp or atphb3 genotype, suggesting the existence of currently unknown signalling cascades. The data show that ANAC017 function extends beyond common retrograde transcriptional responses and affects downstream protein abundance and enzyme activity of alternative oxidase, as well as steady-state energy metabolism in atphb3 plants. Furthermore, detailed growth analysis revealed that ANAC017-dependent retrograde signalling provides benefits for growth and productivity in plants with mitochondrial defects. In conclusion, ANAC017 plays a key role in both biogenic and operational mitochondrial retrograde signalling, and improves plant performance when mitochondrial function is constitutively impaired.
Original language | English |
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Pages (from-to) | 542-558 |
Number of pages | 17 |
Journal | The Plant Journal |
Volume | 88 |
Issue number | 4 |
Early online date | 17 Oct 2016 |
DOIs | |
Publication status | Published - 1 Nov 2016 |
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Dive into the research topics of 'Retrograde signalling caused by heritable mitochondrial dysfunction is partially mediated by ANAC017 and improves plant performance'. Together they form a unique fingerprint.Projects
- 6 Finished
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In touch with the environment: dissecting early tactile responses in plants
Millar, H. (Investigator 01), Singh, K. (Investigator 02) & Van Aken, O. (Investigator 03)
ARC Australian Research Council
1/01/16 → 31/03/19
Project: Research
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ARC Centre of Excellence in Plant Energy Biology 2014 (CPEB2)
Millar, H. (Investigator 01), Pogson, B. (Investigator 02), Tyerman, S. (Investigator 03), Small, I. (Investigator 04), Whelan, J. (Investigator 05), Borevitz, J. (Investigator 06), Lister, R. (Investigator 07), Atkin, O. (Investigator 08) & Munns, R. (Investigator 09)
ARC Australian Research Council
1/01/14 → 31/05/21
Project: Research
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Defence Gene Expression in Arabidopsis Linked to Melabolic Perturbation & Oxidative Signalling via dsr1
Millar, H. (Investigator 01)
ARC Australian Research Council
1/01/13 → 30/06/16
Project: Research