Retention of paternal DNA methylome in the developing zebrafish germline

Ksenia Skvortsova, Katsiaryna Tarbashevich, Martin Stehling, Ryan Lister, Manuel Irimia, Erez Raz, Ozren Bogdanovic

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two waves of DNA methylation reprogramming occur during mammalian embryogenesis; during preimplantation development and during primordial germ cell (PGC) formation. However, it is currently unclear how evolutionarily conserved these processes are. Here we characterise the DNA methylomes of zebrafish PGCs at four developmental stages and identify retention of paternal epigenetic memory, in stark contrast to the findings in mammals. Gene expression profiling of zebrafish PGCs at the same developmental stages revealed that the embryonic germline is defined by a small number of markers that display strong developmental stage-specificity and that are independent of DNA methylation-mediated regulation. We identified promoters that are specifically targeted by DNA methylation in somatic and germline tissues during vertebrate embryogenesis and that are frequently misregulated in human cancers. Together, these detailed methylome and transcriptome maps of the zebrafish germline provide insight into vertebrate DNA methylation reprogramming and enhance our understanding of the relationships between germline fate acquisition and oncogenesis.

Original languageEnglish
Article number3054
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 11 Jul 2019

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Zebrafish
DNA Methylation
methylation
deoxyribonucleic acid
DNA
vertebrates
Embryonic Development
Vertebrates
Mammals
Gene Expression Profiling
Transcriptome
mammals
Epigenomics
Gene expression
Germ Cells
gene expression
Carcinogenesis
markers
Cells
Tissue

Cite this

Skvortsova, K., Tarbashevich, K., Stehling, M., Lister, R., Irimia, M., Raz, E., & Bogdanovic, O. (2019). Retention of paternal DNA methylome in the developing zebrafish germline. Nature Communications, 10(1), [3054]. https://doi.org/10.1038/s41467-019-10895-6
Skvortsova, Ksenia ; Tarbashevich, Katsiaryna ; Stehling, Martin ; Lister, Ryan ; Irimia, Manuel ; Raz, Erez ; Bogdanovic, Ozren. / Retention of paternal DNA methylome in the developing zebrafish germline. In: Nature Communications. 2019 ; Vol. 10, No. 1.
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Skvortsova, K, Tarbashevich, K, Stehling, M, Lister, R, Irimia, M, Raz, E & Bogdanovic, O 2019, 'Retention of paternal DNA methylome in the developing zebrafish germline' Nature Communications, vol. 10, no. 1, 3054. https://doi.org/10.1038/s41467-019-10895-6

Retention of paternal DNA methylome in the developing zebrafish germline. / Skvortsova, Ksenia; Tarbashevich, Katsiaryna; Stehling, Martin; Lister, Ryan; Irimia, Manuel; Raz, Erez; Bogdanovic, Ozren.

In: Nature Communications, Vol. 10, No. 1, 3054, 11.07.2019.

Research output: Contribution to journalArticle

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AU - Skvortsova, Ksenia

AU - Tarbashevich, Katsiaryna

AU - Stehling, Martin

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AU - Raz, Erez

AU - Bogdanovic, Ozren

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