Respiratory viruses in young South African children with acute lower respiratory infections and interactions with HIV

Alicia A. Annamalay, S. Abbott, C. Sikazwe, Siew-Kim Khoo, Joelene Bizzintino, Guicheng Zhang, Ingrid Laing, G.R. Chidlow, David William Smith, J. Gern, Jack Goldblatt, Deborah Lehmann, R.J. Green, Peter N. Le Souef

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Abstract

© 2016 Elsevier B.V.

Background: Human rhinovirus (RV) is the most common respiratory virus and has been associated with frequent and severe acute lower respiratory infections (ALRI). The prevalence of RV species among HIV-infected children in South Africa is unknown. Objectives: To describe the prevalence of respiratory viruses, including RV species, associated with HIV status and other clinical symptoms in children less than two years of age with and without ALRI in Pretoria, South Africa. Study design: Nasopharyngeal aspirates were collected from 105 hospitalized ALRI cases and 53 non-ALRI controls less than two years of age. HIV status was determined. Common respiratory viruses were identified by PCR, and RV species and genotypes were identified by semi-nested PCR, sequencing and phylogenetic tree analyses. Results: Respiratory viruses were more common among ALRI cases than controls (83.8% vs. 69.2%; p = 0.041). RV was the most commonly identified virus in cases with pneumonia (45.6%) or bronchiolitis (52.1%), regardless of HIV status, as well as in controls (39.6%). RV-A was identified in 26.7% of cases and 15.1% of controls while RV-C was identified in 21.0% of cases and 18.9% of controls. HIV-infected children were more likely to be diagnosed with pneumonia than bronchiolitis (p <0.01). RSV was not identified in any HIV-infected cases (n = 15) compared with 30.6% of HIV-uninfected cases (n = 85, p = 0.013), and was identified more frequently in bronchiolitis than in pneumonia cases (43.8% vs. 12.3%; p <0.01). Conclusions: RV-A and RV-C are endemic in South African children and HIV infection may be protective against RSV and bronchiolitis.

Original languageEnglish
Pages (from-to)58-63
JournalJournal of Clinical Virology
Volume81
Early online date4 Jun 2016
DOIs
Publication statusPublished - Aug 2016

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Rhinovirus
Respiratory Tract Infections
HIV
Viruses
Bronchiolitis
Pneumonia
Infection Control
South Africa
Polymerase Chain Reaction
HIV Infections
Genotype

Cite this

Annamalay, Alicia A. ; Abbott, S. ; Sikazwe, C. ; Khoo, Siew-Kim ; Bizzintino, Joelene ; Zhang, Guicheng ; Laing, Ingrid ; Chidlow, G.R. ; Smith, David William ; Gern, J. ; Goldblatt, Jack ; Lehmann, Deborah ; Green, R.J. ; Le Souef, Peter N. / Respiratory viruses in young South African children with acute lower respiratory infections and interactions with HIV. In: Journal of Clinical Virology. 2016 ; Vol. 81. pp. 58-63.
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abstract = "{\circledC} 2016 Elsevier B.V.Background: Human rhinovirus (RV) is the most common respiratory virus and has been associated with frequent and severe acute lower respiratory infections (ALRI). The prevalence of RV species among HIV-infected children in South Africa is unknown. Objectives: To describe the prevalence of respiratory viruses, including RV species, associated with HIV status and other clinical symptoms in children less than two years of age with and without ALRI in Pretoria, South Africa. Study design: Nasopharyngeal aspirates were collected from 105 hospitalized ALRI cases and 53 non-ALRI controls less than two years of age. HIV status was determined. Common respiratory viruses were identified by PCR, and RV species and genotypes were identified by semi-nested PCR, sequencing and phylogenetic tree analyses. Results: Respiratory viruses were more common among ALRI cases than controls (83.8{\%} vs. 69.2{\%}; p = 0.041). RV was the most commonly identified virus in cases with pneumonia (45.6{\%}) or bronchiolitis (52.1{\%}), regardless of HIV status, as well as in controls (39.6{\%}). RV-A was identified in 26.7{\%} of cases and 15.1{\%} of controls while RV-C was identified in 21.0{\%} of cases and 18.9{\%} of controls. HIV-infected children were more likely to be diagnosed with pneumonia than bronchiolitis (p <0.01). RSV was not identified in any HIV-infected cases (n = 15) compared with 30.6{\%} of HIV-uninfected cases (n = 85, p = 0.013), and was identified more frequently in bronchiolitis than in pneumonia cases (43.8{\%} vs. 12.3{\%}; p <0.01). Conclusions: RV-A and RV-C are endemic in South African children and HIV infection may be protective against RSV and bronchiolitis.",
author = "Annamalay, {Alicia A.} and S. Abbott and C. Sikazwe and Siew-Kim Khoo and Joelene Bizzintino and Guicheng Zhang and Ingrid Laing and G.R. Chidlow and Smith, {David William} and J. Gern and Jack Goldblatt and Deborah Lehmann and R.J. Green and {Le Souef}, {Peter N.}",
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Respiratory viruses in young South African children with acute lower respiratory infections and interactions with HIV. / Annamalay, Alicia A.; Abbott, S.; Sikazwe, C.; Khoo, Siew-Kim; Bizzintino, Joelene; Zhang, Guicheng; Laing, Ingrid; Chidlow, G.R.; Smith, David William; Gern, J.; Goldblatt, Jack; Lehmann, Deborah; Green, R.J.; Le Souef, Peter N.

In: Journal of Clinical Virology, Vol. 81, 08.2016, p. 58-63.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Respiratory viruses in young South African children with acute lower respiratory infections and interactions with HIV

AU - Annamalay, Alicia A.

AU - Abbott, S.

AU - Sikazwe, C.

AU - Khoo, Siew-Kim

AU - Bizzintino, Joelene

AU - Zhang, Guicheng

AU - Laing, Ingrid

AU - Chidlow, G.R.

AU - Smith, David William

AU - Gern, J.

AU - Goldblatt, Jack

AU - Lehmann, Deborah

AU - Green, R.J.

AU - Le Souef, Peter N.

PY - 2016/8

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N2 - © 2016 Elsevier B.V.Background: Human rhinovirus (RV) is the most common respiratory virus and has been associated with frequent and severe acute lower respiratory infections (ALRI). The prevalence of RV species among HIV-infected children in South Africa is unknown. Objectives: To describe the prevalence of respiratory viruses, including RV species, associated with HIV status and other clinical symptoms in children less than two years of age with and without ALRI in Pretoria, South Africa. Study design: Nasopharyngeal aspirates were collected from 105 hospitalized ALRI cases and 53 non-ALRI controls less than two years of age. HIV status was determined. Common respiratory viruses were identified by PCR, and RV species and genotypes were identified by semi-nested PCR, sequencing and phylogenetic tree analyses. Results: Respiratory viruses were more common among ALRI cases than controls (83.8% vs. 69.2%; p = 0.041). RV was the most commonly identified virus in cases with pneumonia (45.6%) or bronchiolitis (52.1%), regardless of HIV status, as well as in controls (39.6%). RV-A was identified in 26.7% of cases and 15.1% of controls while RV-C was identified in 21.0% of cases and 18.9% of controls. HIV-infected children were more likely to be diagnosed with pneumonia than bronchiolitis (p <0.01). RSV was not identified in any HIV-infected cases (n = 15) compared with 30.6% of HIV-uninfected cases (n = 85, p = 0.013), and was identified more frequently in bronchiolitis than in pneumonia cases (43.8% vs. 12.3%; p <0.01). Conclusions: RV-A and RV-C are endemic in South African children and HIV infection may be protective against RSV and bronchiolitis.

AB - © 2016 Elsevier B.V.Background: Human rhinovirus (RV) is the most common respiratory virus and has been associated with frequent and severe acute lower respiratory infections (ALRI). The prevalence of RV species among HIV-infected children in South Africa is unknown. Objectives: To describe the prevalence of respiratory viruses, including RV species, associated with HIV status and other clinical symptoms in children less than two years of age with and without ALRI in Pretoria, South Africa. Study design: Nasopharyngeal aspirates were collected from 105 hospitalized ALRI cases and 53 non-ALRI controls less than two years of age. HIV status was determined. Common respiratory viruses were identified by PCR, and RV species and genotypes were identified by semi-nested PCR, sequencing and phylogenetic tree analyses. Results: Respiratory viruses were more common among ALRI cases than controls (83.8% vs. 69.2%; p = 0.041). RV was the most commonly identified virus in cases with pneumonia (45.6%) or bronchiolitis (52.1%), regardless of HIV status, as well as in controls (39.6%). RV-A was identified in 26.7% of cases and 15.1% of controls while RV-C was identified in 21.0% of cases and 18.9% of controls. HIV-infected children were more likely to be diagnosed with pneumonia than bronchiolitis (p <0.01). RSV was not identified in any HIV-infected cases (n = 15) compared with 30.6% of HIV-uninfected cases (n = 85, p = 0.013), and was identified more frequently in bronchiolitis than in pneumonia cases (43.8% vs. 12.3%; p <0.01). Conclusions: RV-A and RV-C are endemic in South African children and HIV infection may be protective against RSV and bronchiolitis.

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DO - 10.1016/j.jcv.2016.06.002

M3 - Article

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SP - 58

EP - 63

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

ER -