Respiratory syncytial virus binds and undergoes transcription in neutrophils from the blood and airways of infants with severe bronchiolitis

Clare P Halfhide; Brian F Flanagan; Stephen P Brearey; John A Hunt; Angela M Fonceca; Paul S McNamara; Deborah Howarth; Steven Edwards; Rosalind L Smyth

doi:10.1093/infdis/jir280

Respiratory syncytial virus binds and undergoes transcription in neutrophils from the blood and airways of infants with severe bronchiolitis

Clare P Halfhide, Brian F Flanagan, Stephen P Brearey, John A Hunt, Angela M Fonceca, Paul S McNamara, Deborah Howarth, Steven Edwards, Rosalind L Smyth

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

BACKGROUND: Neutrophils are the predominant cell in the lung inflammatory infiltrate of infants with respiratory syncytial virus (RSV) bronchiolitis. Although it has previously been shown that neutrophils from both blood and bronchoalveolar lavage (BAL) are activated, little is understood about their role in response to RSV infection. This study investigated whether RSV proteins and mRNA are present in neutrophils from blood and BAL of infected infants.

METHODS: We obtained blood and BAL samples from 20 infants with severe RSV bronchiolitis and 8 healthy control infants. Neutrophil RSV F, G, and N proteins, RSV N genomic RNA, and messenger RNA (mRNA) were quantified.

RESULTS: RSV proteins were found in BAL and blood neutrophils in infants with RSV disease but not in neutrophils from healthy infants. BAL and blood neutrophils from infants with RSV disease, but not those from healthy infants, expressed RSV N genomic RNA, indicating uptake of whole virus; 17 of 20 BAL and 8 of 9 blood neutrophils from patients expressed RSV N mRNA.

CONCLUSIONS: This work shows, for the first time, the presence of RSV proteins and mRNA transcripts within BAL and blood neutrophils from infants with severe RSV bronchiolitis.

Original language	English
Pages (from-to)	451-8
Number of pages	8
Journal	The Journal of infectious diseases
Volume	204
Issue number	3
DOIs	https://doi.org/10.1093/infdis/jir280
Publication status	Published - 1 Aug 2011
Externally published	Yes

Access to Document

10.1093/infdis/jir280

Cite this

Halfhide, C. P., Flanagan, B. F., Brearey, S. P., Hunt, J. A., Fonceca, A. M., McNamara, P. S., Howarth, D., Edwards, S., & Smyth, R. L. (2011). Respiratory syncytial virus binds and undergoes transcription in neutrophils from the blood and airways of infants with severe bronchiolitis. The Journal of infectious diseases, 204(3), 451-8. https://doi.org/10.1093/infdis/jir280

@article{1db69432593644d989c26cf8fbd6073e,

title = "Respiratory syncytial virus binds and undergoes transcription in neutrophils from the blood and airways of infants with severe bronchiolitis",

abstract = "BACKGROUND: Neutrophils are the predominant cell in the lung inflammatory infiltrate of infants with respiratory syncytial virus (RSV) bronchiolitis. Although it has previously been shown that neutrophils from both blood and bronchoalveolar lavage (BAL) are activated, little is understood about their role in response to RSV infection. This study investigated whether RSV proteins and mRNA are present in neutrophils from blood and BAL of infected infants.METHODS: We obtained blood and BAL samples from 20 infants with severe RSV bronchiolitis and 8 healthy control infants. Neutrophil RSV F, G, and N proteins, RSV N genomic RNA, and messenger RNA (mRNA) were quantified.RESULTS: RSV proteins were found in BAL and blood neutrophils in infants with RSV disease but not in neutrophils from healthy infants. BAL and blood neutrophils from infants with RSV disease, but not those from healthy infants, expressed RSV N genomic RNA, indicating uptake of whole virus; 17 of 20 BAL and 8 of 9 blood neutrophils from patients expressed RSV N mRNA.CONCLUSIONS: This work shows, for the first time, the presence of RSV proteins and mRNA transcripts within BAL and blood neutrophils from infants with severe RSV bronchiolitis.",

keywords = "Bronchiolitis, Viral/immunology, Bronchoalveolar Lavage Fluid/virology, Female, Humans, Infant, Infant, Newborn, Male, Neutrophils/physiology, RNA, Messenger/analysis, Respiratory Syncytial Viruses/genetics, Viral Fusion Proteins/analysis",

author = "Halfhide, \{Clare P\} and Flanagan, \{Brian F\} and Brearey, \{Stephen P\} and Hunt, \{John A\} and Fonceca, \{Angela M\} and McNamara, \{Paul S\} and Deborah Howarth and Steven Edwards and Smyth, \{Rosalind L\}",

year = "2011",

month = aug,

day = "1",

doi = "10.1093/infdis/jir280",

language = "English",

volume = "204",

pages = "451--8",

journal = "The Journal of infectious diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Respiratory syncytial virus binds and undergoes transcription in neutrophils from the blood and airways of infants with severe bronchiolitis

AU - Halfhide, Clare P

AU - Flanagan, Brian F

AU - Brearey, Stephen P

AU - Hunt, John A

AU - Fonceca, Angela M

AU - McNamara, Paul S

AU - Howarth, Deborah

AU - Edwards, Steven

AU - Smyth, Rosalind L

PY - 2011/8/1

Y1 - 2011/8/1

N2 - BACKGROUND: Neutrophils are the predominant cell in the lung inflammatory infiltrate of infants with respiratory syncytial virus (RSV) bronchiolitis. Although it has previously been shown that neutrophils from both blood and bronchoalveolar lavage (BAL) are activated, little is understood about their role in response to RSV infection. This study investigated whether RSV proteins and mRNA are present in neutrophils from blood and BAL of infected infants.METHODS: We obtained blood and BAL samples from 20 infants with severe RSV bronchiolitis and 8 healthy control infants. Neutrophil RSV F, G, and N proteins, RSV N genomic RNA, and messenger RNA (mRNA) were quantified.RESULTS: RSV proteins were found in BAL and blood neutrophils in infants with RSV disease but not in neutrophils from healthy infants. BAL and blood neutrophils from infants with RSV disease, but not those from healthy infants, expressed RSV N genomic RNA, indicating uptake of whole virus; 17 of 20 BAL and 8 of 9 blood neutrophils from patients expressed RSV N mRNA.CONCLUSIONS: This work shows, for the first time, the presence of RSV proteins and mRNA transcripts within BAL and blood neutrophils from infants with severe RSV bronchiolitis.

AB - BACKGROUND: Neutrophils are the predominant cell in the lung inflammatory infiltrate of infants with respiratory syncytial virus (RSV) bronchiolitis. Although it has previously been shown that neutrophils from both blood and bronchoalveolar lavage (BAL) are activated, little is understood about their role in response to RSV infection. This study investigated whether RSV proteins and mRNA are present in neutrophils from blood and BAL of infected infants.METHODS: We obtained blood and BAL samples from 20 infants with severe RSV bronchiolitis and 8 healthy control infants. Neutrophil RSV F, G, and N proteins, RSV N genomic RNA, and messenger RNA (mRNA) were quantified.RESULTS: RSV proteins were found in BAL and blood neutrophils in infants with RSV disease but not in neutrophils from healthy infants. BAL and blood neutrophils from infants with RSV disease, but not those from healthy infants, expressed RSV N genomic RNA, indicating uptake of whole virus; 17 of 20 BAL and 8 of 9 blood neutrophils from patients expressed RSV N mRNA.CONCLUSIONS: This work shows, for the first time, the presence of RSV proteins and mRNA transcripts within BAL and blood neutrophils from infants with severe RSV bronchiolitis.

KW - Bronchiolitis, Viral/immunology

KW - Bronchoalveolar Lavage Fluid/virology

KW - Female

KW - Humans

KW - Infant

KW - Infant, Newborn

KW - Male

KW - Neutrophils/physiology

KW - RNA, Messenger/analysis

KW - Respiratory Syncytial Viruses/genetics

KW - Viral Fusion Proteins/analysis

UR - https://www.scopus.com/pages/publications/79960139777

U2 - 10.1093/infdis/jir280

DO - 10.1093/infdis/jir280

M3 - Article

C2 - 21742845

SN - 0022-1899

VL - 204

SP - 451

EP - 458

JO - The Journal of infectious diseases

JF - The Journal of infectious diseases

IS - 3

ER -

Respiratory syncytial virus binds and undergoes transcription in neutrophils from the blood and airways of infants with severe bronchiolitis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this