Resolving intergeneric relationships in the aervoid clade and the backbone of Ptilotus (Amaranthaceae): Evidence from whole plastid genomes and morphology

Timothy A. Hammer, Xiao Zhong, Catherine Colas des Francs-Small, Paul G. Nevill, Ian D. Small, Kevin R. Thiele

Research output: Contribution to journalArticle

1 Downloads (Pure)

Abstract

The informally named “aervoid clade” in Amaranthaceae includes ~134 species in five genera: Ptilotus (~120 spp.), Aerva (11 spp.) and the monotypic Nothosaerva, Omegandra, and Kelita. The relationships of the small aervoid genera to the large genus Ptilotus, and relationships between major clades within Ptilotus, are poorly resolved. The aims of this study were to: (1) elucidate relationships between genera and within Ptilotus using a phylogenomic approach; (2) identify morphological characters within each genus to help delimit generic boundaries; and (3) provide an updated taxonomic framework for the aervoids. A well-supported coding DNA sequence (CDS) phylogeny was constructed for 36 aervoid and 5 outgroup species based on 69 gene sequences derived from assembled whole-plastid genomes. The CDS tree was used to constrain relationships on a larger phylogeny based on Sanger-sequenced ITS and matK for 135 taxa, comprising near-comprehensive sampling within the aervoids. Both datasets were analysed using maximum likelihood and Bayesian inference. Morphological characters were assessed from herbarium specimens. Our study demonstrates that Aerva is polyphyletic; this is resolved by reinstating Ouret and erecting a new genus, Paraerva. Kelita is found to be deeply nested within Ptilotus and is formally synonymised. The well-resolved phylogeny of Ptilotus presented here will inform future studies in biogeography and character evolution. A taxonomic treatment is provided for all aervoid genera, and new combinations are made.

Original languageEnglish
Pages (from-to)297-314
JournalTaxon
Volume68
Issue number2
DOIs
Publication statusPublished - 1 Apr 2019

Fingerprint

Amaranthaceae
plastid
Aerva
phylogeny
genome
nucleotide sequences
DNA
herbarium
herbaria
biogeography
new combination
new genus
gene
sampling
plastid genome

Cite this

@article{b25f2d73fb25425fb90e0c4db5343c50,
title = "Resolving intergeneric relationships in the aervoid clade and the backbone of Ptilotus (Amaranthaceae): Evidence from whole plastid genomes and morphology",
abstract = "The informally named “aervoid clade” in Amaranthaceae includes ~134 species in five genera: Ptilotus (~120 spp.), Aerva (11 spp.) and the monotypic Nothosaerva, Omegandra, and Kelita. The relationships of the small aervoid genera to the large genus Ptilotus, and relationships between major clades within Ptilotus, are poorly resolved. The aims of this study were to: (1) elucidate relationships between genera and within Ptilotus using a phylogenomic approach; (2) identify morphological characters within each genus to help delimit generic boundaries; and (3) provide an updated taxonomic framework for the aervoids. A well-supported coding DNA sequence (CDS) phylogeny was constructed for 36 aervoid and 5 outgroup species based on 69 gene sequences derived from assembled whole-plastid genomes. The CDS tree was used to constrain relationships on a larger phylogeny based on Sanger-sequenced ITS and matK for 135 taxa, comprising near-comprehensive sampling within the aervoids. Both datasets were analysed using maximum likelihood and Bayesian inference. Morphological characters were assessed from herbarium specimens. Our study demonstrates that Aerva is polyphyletic; this is resolved by reinstating Ouret and erecting a new genus, Paraerva. Kelita is found to be deeply nested within Ptilotus and is formally synonymised. The well-resolved phylogeny of Ptilotus presented here will inform future studies in biogeography and character evolution. A taxonomic treatment is provided for all aervoid genera, and new combinations are made.",
keywords = "Amaranthaceae, Caryophyllales, new genus, nomenclature, phylogenomics, Ptilotus, taxonomy",
author = "Hammer, {Timothy A.} and Xiao Zhong and {Colas des Francs-Small}, Catherine and Nevill, {Paul G.} and Small, {Ian D.} and Thiele, {Kevin R.}",
year = "2019",
month = "4",
day = "1",
doi = "10.1002/tax.12054",
language = "English",
volume = "68",
pages = "297--314",
journal = "Taxon",
issn = "0040-0262",
publisher = "International Association for Plant Taxonomy",
number = "2",

}

TY - JOUR

T1 - Resolving intergeneric relationships in the aervoid clade and the backbone of Ptilotus (Amaranthaceae)

T2 - Evidence from whole plastid genomes and morphology

AU - Hammer, Timothy A.

AU - Zhong, Xiao

AU - Colas des Francs-Small, Catherine

AU - Nevill, Paul G.

AU - Small, Ian D.

AU - Thiele, Kevin R.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - The informally named “aervoid clade” in Amaranthaceae includes ~134 species in five genera: Ptilotus (~120 spp.), Aerva (11 spp.) and the monotypic Nothosaerva, Omegandra, and Kelita. The relationships of the small aervoid genera to the large genus Ptilotus, and relationships between major clades within Ptilotus, are poorly resolved. The aims of this study were to: (1) elucidate relationships between genera and within Ptilotus using a phylogenomic approach; (2) identify morphological characters within each genus to help delimit generic boundaries; and (3) provide an updated taxonomic framework for the aervoids. A well-supported coding DNA sequence (CDS) phylogeny was constructed for 36 aervoid and 5 outgroup species based on 69 gene sequences derived from assembled whole-plastid genomes. The CDS tree was used to constrain relationships on a larger phylogeny based on Sanger-sequenced ITS and matK for 135 taxa, comprising near-comprehensive sampling within the aervoids. Both datasets were analysed using maximum likelihood and Bayesian inference. Morphological characters were assessed from herbarium specimens. Our study demonstrates that Aerva is polyphyletic; this is resolved by reinstating Ouret and erecting a new genus, Paraerva. Kelita is found to be deeply nested within Ptilotus and is formally synonymised. The well-resolved phylogeny of Ptilotus presented here will inform future studies in biogeography and character evolution. A taxonomic treatment is provided for all aervoid genera, and new combinations are made.

AB - The informally named “aervoid clade” in Amaranthaceae includes ~134 species in five genera: Ptilotus (~120 spp.), Aerva (11 spp.) and the monotypic Nothosaerva, Omegandra, and Kelita. The relationships of the small aervoid genera to the large genus Ptilotus, and relationships between major clades within Ptilotus, are poorly resolved. The aims of this study were to: (1) elucidate relationships between genera and within Ptilotus using a phylogenomic approach; (2) identify morphological characters within each genus to help delimit generic boundaries; and (3) provide an updated taxonomic framework for the aervoids. A well-supported coding DNA sequence (CDS) phylogeny was constructed for 36 aervoid and 5 outgroup species based on 69 gene sequences derived from assembled whole-plastid genomes. The CDS tree was used to constrain relationships on a larger phylogeny based on Sanger-sequenced ITS and matK for 135 taxa, comprising near-comprehensive sampling within the aervoids. Both datasets were analysed using maximum likelihood and Bayesian inference. Morphological characters were assessed from herbarium specimens. Our study demonstrates that Aerva is polyphyletic; this is resolved by reinstating Ouret and erecting a new genus, Paraerva. Kelita is found to be deeply nested within Ptilotus and is formally synonymised. The well-resolved phylogeny of Ptilotus presented here will inform future studies in biogeography and character evolution. A taxonomic treatment is provided for all aervoid genera, and new combinations are made.

KW - Amaranthaceae

KW - Caryophyllales

KW - new genus

KW - nomenclature

KW - phylogenomics

KW - Ptilotus

KW - taxonomy

UR - http://www.scopus.com/inward/record.url?scp=85068934414&partnerID=8YFLogxK

U2 - 10.1002/tax.12054

DO - 10.1002/tax.12054

M3 - Article

VL - 68

SP - 297

EP - 314

JO - Taxon

JF - Taxon

SN - 0040-0262

IS - 2

ER -