Renal oxygenation in acute renal ischemia-reperfusion injury

Amany Abdelkader, Julie Ho, Connie P.C. Ow, Gabriela A. Eppel, Niwanthi W. Rajapakse, Markus P. Schlaich, Roger G. Evans

Research output: Contribution to journalArticle

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Abstract

Tissue hypoxia has been demonstrated, in both the renal cortex and medulla, during the acute phase of reperfusion after ischemia induced by occlusion of the aorta upstream from the kidney. However, there are also recent clinical observations indicating relatively well preserved oxygenation in the nonfunctional transplanted kidney. To test whether severe acute kidney injury can occur in the absence of widespread renal tissue hypoxia, we measured cortical and inner medullary tissue PO2 as well as total renal O2 delivery (DO2) and O2 consumption (V ̇O2) during the first 2 h of reperfusion after 60 min of occlusion of the renal artery in anesthetized rats. To perform this experiment, we used a new method for measuring kidney DO2 andV ̇O2 that relies on implantation of fluorescence optodes in the femoral artery and renal vein. We were unable to detect reductions in renal cortical or inner medullary tissue PO2 during reperfusion after ischemia localized to the kidney. This is likely explained by the observation thatV ̇O2 (-57%) was reduced by at least as much as DO2 (-45%), due to a large reduction in glomerular filtration (-94%). However, localized tissue hypoxia, as evidence by pimonidazole adduct immunohistochemistry, was detected in kidneys subjected to ischemia and reperfusion, particularly in, but not exclusive to, the outer medulla. Thus, cellular hypoxia, particularly in the outer medulla, may still be present during reperfusion even when reductions in tissue PO2 are not detected in the cortex or inner medulla.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume306
Issue number9
DOIs
Publication statusPublished - 1 May 2014
Externally publishedYes

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Reperfusion Injury
Kidney
Reperfusion
Ischemia
Cell Hypoxia
Renal Veins
Femoral Vein
Renal Artery
Femoral Artery
Acute Kidney Injury
Aorta
Fluorescence
Immunohistochemistry
Observation

Cite this

Abdelkader, Amany ; Ho, Julie ; Ow, Connie P.C. ; Eppel, Gabriela A. ; Rajapakse, Niwanthi W. ; Schlaich, Markus P. ; Evans, Roger G. / Renal oxygenation in acute renal ischemia-reperfusion injury. In: American Journal of Physiology - Renal Physiology. 2014 ; Vol. 306, No. 9.
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Renal oxygenation in acute renal ischemia-reperfusion injury. / Abdelkader, Amany; Ho, Julie; Ow, Connie P.C.; Eppel, Gabriela A.; Rajapakse, Niwanthi W.; Schlaich, Markus P.; Evans, Roger G.

In: American Journal of Physiology - Renal Physiology, Vol. 306, No. 9, 01.05.2014.

Research output: Contribution to journalArticle

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T1 - Renal oxygenation in acute renal ischemia-reperfusion injury

AU - Abdelkader, Amany

AU - Ho, Julie

AU - Ow, Connie P.C.

AU - Eppel, Gabriela A.

AU - Rajapakse, Niwanthi W.

AU - Schlaich, Markus P.

AU - Evans, Roger G.

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AB - Tissue hypoxia has been demonstrated, in both the renal cortex and medulla, during the acute phase of reperfusion after ischemia induced by occlusion of the aorta upstream from the kidney. However, there are also recent clinical observations indicating relatively well preserved oxygenation in the nonfunctional transplanted kidney. To test whether severe acute kidney injury can occur in the absence of widespread renal tissue hypoxia, we measured cortical and inner medullary tissue PO2 as well as total renal O2 delivery (DO2) and O2 consumption (V ̇O2) during the first 2 h of reperfusion after 60 min of occlusion of the renal artery in anesthetized rats. To perform this experiment, we used a new method for measuring kidney DO2 andV ̇O2 that relies on implantation of fluorescence optodes in the femoral artery and renal vein. We were unable to detect reductions in renal cortical or inner medullary tissue PO2 during reperfusion after ischemia localized to the kidney. This is likely explained by the observation thatV ̇O2 (-57%) was reduced by at least as much as DO2 (-45%), due to a large reduction in glomerular filtration (-94%). However, localized tissue hypoxia, as evidence by pimonidazole adduct immunohistochemistry, was detected in kidneys subjected to ischemia and reperfusion, particularly in, but not exclusive to, the outer medulla. Thus, cellular hypoxia, particularly in the outer medulla, may still be present during reperfusion even when reductions in tissue PO2 are not detected in the cortex or inner medulla.

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