Remineralization of initial enamel carious lesions using fluoridated milk in vitro

Kristine L. Ongtenco, Robert P. Anthonappa, Anut Itthagarun, Nigel M. King, Ratilal Lalloo, Raj G. Nair

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


OBJECTIVES: Milk is a universal dietary component and it is now recognized as an effective medium for the delivery of fluoride (F). This study sought (i) to evaluate fluoridated milk (2.5 ppm, 5 ppm, 10 ppm) for remineralizing carious lesions and (ii) to determine the optimum frequency for treating carious lesions with fluoridated milk.

MATERIALS AND METHODS: Artificial carious lesions, 90-180 μm deep, were created on extracted third molar teeth that were sectioned to produce specimens of 100-120 μm thickness. Specimens were randomly divided into 13 groups (n = 20) for treatment with deionized water, plain milk or fluoridated milk (2.5 ppm, 5 ppm, 10 ppm); once daily, twice daily or on alternate days as part of a 20-day pH cycling model. Lesion depth (LD) and mineral content were evaluated before and after pH cycling. Paired t-test, ANOVA and Student-Newman-Keuls tests were employed to make comparisons within and between the different groups.

RESULTS: Fluoridated milk significantly reduced LD and increased the mineral content of the lesions compared to plain milk and deionized water (p < 0.05). The greatest reduction in LD was with 2.5 ppm F milk used twice daily (p < 0.05).

CONCLUSIONS: Milk with 2.5 ppm F used twice daily demonstrated the greatest remineralization of artificial enamel carious lesions in vitro.

Original languageEnglish
Pages (from-to)737-744
Number of pages8
JournalActa Odontologica Scandinavica
Issue number8
Publication statusPublished - 1 Nov 2014
Externally publishedYes


Dive into the research topics of 'Remineralization of initial enamel carious lesions using fluoridated milk in vitro'. Together they form a unique fingerprint.

Cite this