Regulation of uncoupled respiratory electron transport in plants

Ruth Clare Holtzapffel

doi:10.26182/5d524d40ff5ed

Regulation of uncoupled respiratory electron transport in plants

Ruth Clare Holtzapffel

Research output: Thesis › Doctoral Thesis

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Abstract

[Truncated] Plant mitochondria contain a branched respiratory electron transport chain that terminates in two terminal oxidases, the cytochrome oxidase and the alternative oxidase (AOX). Electron transport through the cytochrome oxidase pathway results in the formation of a proton motive force that can be used to drive ATP synthesis. AOX, however, directly oxidises ubiquinol and thus bypasses two proton translocation sites. This pathway mediates non-coupled respiratory electron transport. Plant mitochondria also contain an uncoupling protein (UCP), which dissipates the proton motive force as it is generated, resulting in uncoupled respiratory electron transport. This project aimed to investigate the coordination of expression of the UCP and AOX proteins as well as the role of a highly conserved cysteine residue in the regulation of AOX activity.
The coordination of UCP and AOX protein expression was investigated in ripening and chilled tomato (Lycopersicon esculentum L.) fruit, two situations that have previously been shown to induce AOX expression in other species. The peak in expression of both proteins was found to occur after the respiratory climacteric in tomato. The abundance of bothproteins was also found to increase in response to chilling of mature green tomatoes. A multigene family for AOX was cloned from a tomato fruit cDNA library.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
DOIs	https://doi.org/10.26182/5d524d40ff5ed
Publication status	Unpublished - 2002

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title = "Regulation of uncoupled respiratory electron transport in plants",

abstract = "[Truncated] Plant mitochondria contain a branched respiratory electron transport chain that terminates in two terminal oxidases, the cytochrome oxidase and the alternative oxidase (AOX). Electron transport through the cytochrome oxidase pathway results in the formation of a proton motive force that can be used to drive ATP synthesis. AOX, however, directly oxidises ubiquinol and thus bypasses two proton translocation sites. This pathway mediates non-coupled respiratory electron transport. Plant mitochondria also contain an uncoupling protein (UCP), which dissipates the proton motive force as it is generated, resulting in uncoupled respiratory electron transport. This project aimed to investigate the coordination of expression of the UCP and AOX proteins as well as the role of a highly conserved cysteine residue in the regulation of AOX activity.The coordination of UCP and AOX protein expression was investigated in ripening and chilled tomato (Lycopersicon esculentum L.) fruit, two situations that have previously been shown to induce AOX expression in other species. The peak in expression of both proteins was found to occur after the respiratory climacteric in tomato. The abundance of bothproteins was also found to increase in response to chilling of mature green tomatoes. A multigene family for AOX was cloned from a tomato fruit cDNA library.",

author = "Holtzapffel, {Ruth Clare}",

note = "This thesis has been made available in the UWA Profiles and Research Repository as part of a UWA Library project to digitise and make available theses completed before 2003. If you are the author of this thesis and would like it removed from the UWA Profiles and Research Repository, please contact digitaltheses-lib@uwa.edu.au",

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PY - 2002

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N2 - [Truncated] Plant mitochondria contain a branched respiratory electron transport chain that terminates in two terminal oxidases, the cytochrome oxidase and the alternative oxidase (AOX). Electron transport through the cytochrome oxidase pathway results in the formation of a proton motive force that can be used to drive ATP synthesis. AOX, however, directly oxidises ubiquinol and thus bypasses two proton translocation sites. This pathway mediates non-coupled respiratory electron transport. Plant mitochondria also contain an uncoupling protein (UCP), which dissipates the proton motive force as it is generated, resulting in uncoupled respiratory electron transport. This project aimed to investigate the coordination of expression of the UCP and AOX proteins as well as the role of a highly conserved cysteine residue in the regulation of AOX activity.The coordination of UCP and AOX protein expression was investigated in ripening and chilled tomato (Lycopersicon esculentum L.) fruit, two situations that have previously been shown to induce AOX expression in other species. The peak in expression of both proteins was found to occur after the respiratory climacteric in tomato. The abundance of bothproteins was also found to increase in response to chilling of mature green tomatoes. A multigene family for AOX was cloned from a tomato fruit cDNA library.

AB - [Truncated] Plant mitochondria contain a branched respiratory electron transport chain that terminates in two terminal oxidases, the cytochrome oxidase and the alternative oxidase (AOX). Electron transport through the cytochrome oxidase pathway results in the formation of a proton motive force that can be used to drive ATP synthesis. AOX, however, directly oxidises ubiquinol and thus bypasses two proton translocation sites. This pathway mediates non-coupled respiratory electron transport. Plant mitochondria also contain an uncoupling protein (UCP), which dissipates the proton motive force as it is generated, resulting in uncoupled respiratory electron transport. This project aimed to investigate the coordination of expression of the UCP and AOX proteins as well as the role of a highly conserved cysteine residue in the regulation of AOX activity.The coordination of UCP and AOX protein expression was investigated in ripening and chilled tomato (Lycopersicon esculentum L.) fruit, two situations that have previously been shown to induce AOX expression in other species. The peak in expression of both proteins was found to occur after the respiratory climacteric in tomato. The abundance of bothproteins was also found to increase in response to chilling of mature green tomatoes. A multigene family for AOX was cloned from a tomato fruit cDNA library.

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