Regulation of human PAX6 expression by miR-7

M. Needhamsen, Robert White, K.M. Giles, Sarah Dunlop, Meghan Thomas

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    The paired box gene 6 (PAX6) is a powerful mediator of eye and brain organogenesis whose spatiotemporal expression is exquisitely controlled by multiple mechanisms, including post-transcriptional regulation by microRNAs (miRNAs). In the present study, we use bioinformatic predictions to identify three candidate microRNA-7 (miR-7) target sites in the human PAX6 3′ untranslated region (3′-UTR) and demonstrate that two of them are functionally active in a human cell line. Furthermore, transient transfection of cells with synthetic miR-7 inhibits PAX6 protein expression but does not alter levels of PAX6 mRNA, suggesting that miR-7 induces translational repression of PAX6. Finally, a comparison of PAX6 3′-UTRs across species reveals that one of the functional miR-7 target sites is conserved, whereas the second functional target site is found only in primates. Thus, the interaction between PAX6 and miR-7 appears to be highly conserved; however, the precise number of sites through which this interaction occurs may have expanded throughout evolution. © the authors, publisher and licensee Libertas Academica Limited.
    Original languageEnglish
    Pages (from-to)107-113
    JournalEvolutionary Bioinformatics
    Volume10
    DOIs
    Publication statusPublished - 14 Jul 2014

    Fingerprint Dive into the research topics of 'Regulation of human PAX6 expression by miR-7'. Together they form a unique fingerprint.

  • Cite this