Regulation of blood-brain barrier by a subset of astrocytes through mitochondrial transfer

Delin Liu

doi:10.26182/91k1-gh90

Regulation of blood-brain barrier by a subset of astrocytes through mitochondrial transfer

Delin Liu

UWA Medical School

Research output: Thesis › Doctoral Thesis

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Abstract

The blood-brain barrier (BBB) acts as the crucial protective structure in the central nervous system(CNS). Here we showed that a subset of astrocytes marked by Dmp1 expression transfer mitochondria to endothelial cells via their end feet for maintaining the integrity of BBB. Deletion of Mitofusion 2 (Mfn2) in Dmp1-expressing astrocytes inhibited the mitochondrial transfer and caused BBB leakage. Together, we describe a mechanism in which astrocytes regulate BBB integrity through mitochondrial transfer. Our findings provide new insights into the cellular framework that underpins progressive breakdown of BBB associated with aging and disease.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Zheng, Minghao, Supervisor Gao, Junjie, Supervisor
Award date	1 Jun 2023
DOIs	https://doi.org/10.26182/91k1-gh90
Publication status	Unpublished - 2023

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10.26182/91k1-gh90

THESIS - DOCTOR OF PHILOSOPHY - DELIN Liu - 2023
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Intercellular mitochondrial transfer as a means of tissue revitalization
Liu, D., Gao, Y., Liu, J., Huang, Y., Yin, J., Feng, Y., Shi, L., Meloni, B. P., Zhang, C., Zheng, M. & Gao, J., Dec 2021, In: Signal Transduction and Targeted Therapy. 6, 1, 65.
Research output: Contribution to journal › Review article › peer-review

Open Access
310 Link opens in a new tab Citations (Scopus)
Endoplasmic reticulum mediates mitochondrial transfer within the osteocyte dendritic network
Gao, J., Qin, A., Liu, D., Ruan, R., Wang, Q., Yuan, J., Cheng, T. S., Filipovska, A., Papadimitriou, J. M., Dai, K., Jiang, Q., Gao, X., Feng, J. Q., Takayanagi, H., Zhang, C. & Zheng, M. H., 20 Nov 2019, In: Science Advances. 5, 11, eaaw7215.
Research output: Contribution to journal › Article › peer-review

Open Access
97 Link opens in a new tab Citations (Scopus)

Cite this

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title = "Regulation of blood-brain barrier by a subset of astrocytes through mitochondrial transfer",

abstract = "The blood-brain barrier (BBB) acts as the crucial protective structure in the central nervous system(CNS). Here we showed that a subset of astrocytes marked by Dmp1 expression transfer mitochondria to endothelial cells via their end feet for maintaining the integrity of BBB. Deletion of Mitofusion 2 (Mfn2) in Dmp1-expressing astrocytes inhibited the mitochondrial transfer and caused BBB leakage. Together, we describe a mechanism in which astrocytes regulate BBB integrity through mitochondrial transfer. Our findings provide new insights into the cellular framework that underpins progressive breakdown of BBB associated with aging and disease.",

keywords = "blood-brain barrier, astrocytes, endothelial cells, mitofusin 2, dentin matrix protein 1, mitochondrial transfer",

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language = "English",

school = "The University of Western Australia",

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AB - The blood-brain barrier (BBB) acts as the crucial protective structure in the central nervous system(CNS). Here we showed that a subset of astrocytes marked by Dmp1 expression transfer mitochondria to endothelial cells via their end feet for maintaining the integrity of BBB. Deletion of Mitofusion 2 (Mfn2) in Dmp1-expressing astrocytes inhibited the mitochondrial transfer and caused BBB leakage. Together, we describe a mechanism in which astrocytes regulate BBB integrity through mitochondrial transfer. Our findings provide new insights into the cellular framework that underpins progressive breakdown of BBB associated with aging and disease.

KW - blood-brain barrier

KW - astrocytes

KW - endothelial cells

KW - mitofusin 2

KW - dentin matrix protein 1

KW - mitochondrial transfer

U2 - 10.26182/91k1-gh90

DO - 10.26182/91k1-gh90

M3 - Doctoral Thesis

ER -

Regulation of blood-brain barrier by a subset of astrocytes through mitochondrial transfer

Abstract

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Research output

Intercellular mitochondrial transfer as a means of tissue revitalization

Endoplasmic reticulum mediates mitochondrial transfer within the osteocyte dendritic network

Cite this