Regulation of a novel Fusarium cytokinin in Fusarium pseudograminearum

Ailisa Blum, Aurelie H. Benfield, Jens L. Sorensen, Mikkel R. Nielsen, Simone Bachleitner, Lena Studt, Giovanni Beccari, Lorenzo Covarelli, Jacqueline Batley, Donald M. Gardiner

Research output: Contribution to journalArticle

Abstract

Fusarium pseudograminearum is an agronomically important fungus, which infects many crop plants, including wheat, where it causes Fusarium crown rot. Like many other fungi, the Fusarium genus produces a wide range of secondary metabolites of which only few have been characterized. Recently a novel gene cluster was discovered in F. pseudograminearum, which encodes production of cytokinin-like metabolites collectively named Fusarium cytokinins. They are structurally similar to plant cytokinins and can activate cytokinin signalling in vitro and in planta. Here, the regulation of Fusarium cytokinin production was analysed in vitro. This revealed that, similar to deoxynivalenol (DON) production in Fusarium graminearum, cytokinin production can be induced in vitro by specific nitrogen sources in a pH-dependent manner. DON production was also induced in both F. graminearum and F. pseudograminearum in cytokinin-inducing conditions. In addition, microscopic analyses of wheat seedlings infected with a F. pseudograminearum cytokinin reporter strain showed that the fungus specifically induces its cytokinin production in hyphae, which are in close association with the plant, suggestive of a function of Fusarium cytokinins during infection. Crown Copyright (C) 2019 Published by Elsevier Ltd on behalf of British Mycological Society. All rights reserved.

Original languageEnglish
Pages (from-to)255-266
Number of pages12
JournalFungal Biology
Volume123
Issue number3
DOIs
Publication statusPublished - Mar 2019

Cite this

Blum, A., Benfield, A. H., Sorensen, J. L., Nielsen, M. R., Bachleitner, S., Studt, L., ... Gardiner, D. M. (2019). Regulation of a novel Fusarium cytokinin in Fusarium pseudograminearum. Fungal Biology, 123(3), 255-266. https://doi.org/10.1016/j.funbio.2018.12.009
Blum, Ailisa ; Benfield, Aurelie H. ; Sorensen, Jens L. ; Nielsen, Mikkel R. ; Bachleitner, Simone ; Studt, Lena ; Beccari, Giovanni ; Covarelli, Lorenzo ; Batley, Jacqueline ; Gardiner, Donald M. / Regulation of a novel Fusarium cytokinin in Fusarium pseudograminearum. In: Fungal Biology. 2019 ; Vol. 123, No. 3. pp. 255-266.
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Blum, A, Benfield, AH, Sorensen, JL, Nielsen, MR, Bachleitner, S, Studt, L, Beccari, G, Covarelli, L, Batley, J & Gardiner, DM 2019, 'Regulation of a novel Fusarium cytokinin in Fusarium pseudograminearum' Fungal Biology, vol. 123, no. 3, pp. 255-266. https://doi.org/10.1016/j.funbio.2018.12.009

Regulation of a novel Fusarium cytokinin in Fusarium pseudograminearum. / Blum, Ailisa; Benfield, Aurelie H.; Sorensen, Jens L.; Nielsen, Mikkel R.; Bachleitner, Simone; Studt, Lena; Beccari, Giovanni; Covarelli, Lorenzo; Batley, Jacqueline; Gardiner, Donald M.

In: Fungal Biology, Vol. 123, No. 3, 03.2019, p. 255-266.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Regulation of a novel Fusarium cytokinin in Fusarium pseudograminearum

AU - Blum, Ailisa

AU - Benfield, Aurelie H.

AU - Sorensen, Jens L.

AU - Nielsen, Mikkel R.

AU - Bachleitner, Simone

AU - Studt, Lena

AU - Beccari, Giovanni

AU - Covarelli, Lorenzo

AU - Batley, Jacqueline

AU - Gardiner, Donald M.

PY - 2019/3

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N2 - Fusarium pseudograminearum is an agronomically important fungus, which infects many crop plants, including wheat, where it causes Fusarium crown rot. Like many other fungi, the Fusarium genus produces a wide range of secondary metabolites of which only few have been characterized. Recently a novel gene cluster was discovered in F. pseudograminearum, which encodes production of cytokinin-like metabolites collectively named Fusarium cytokinins. They are structurally similar to plant cytokinins and can activate cytokinin signalling in vitro and in planta. Here, the regulation of Fusarium cytokinin production was analysed in vitro. This revealed that, similar to deoxynivalenol (DON) production in Fusarium graminearum, cytokinin production can be induced in vitro by specific nitrogen sources in a pH-dependent manner. DON production was also induced in both F. graminearum and F. pseudograminearum in cytokinin-inducing conditions. In addition, microscopic analyses of wheat seedlings infected with a F. pseudograminearum cytokinin reporter strain showed that the fungus specifically induces its cytokinin production in hyphae, which are in close association with the plant, suggestive of a function of Fusarium cytokinins during infection. Crown Copyright (C) 2019 Published by Elsevier Ltd on behalf of British Mycological Society. All rights reserved.

AB - Fusarium pseudograminearum is an agronomically important fungus, which infects many crop plants, including wheat, where it causes Fusarium crown rot. Like many other fungi, the Fusarium genus produces a wide range of secondary metabolites of which only few have been characterized. Recently a novel gene cluster was discovered in F. pseudograminearum, which encodes production of cytokinin-like metabolites collectively named Fusarium cytokinins. They are structurally similar to plant cytokinins and can activate cytokinin signalling in vitro and in planta. Here, the regulation of Fusarium cytokinin production was analysed in vitro. This revealed that, similar to deoxynivalenol (DON) production in Fusarium graminearum, cytokinin production can be induced in vitro by specific nitrogen sources in a pH-dependent manner. DON production was also induced in both F. graminearum and F. pseudograminearum in cytokinin-inducing conditions. In addition, microscopic analyses of wheat seedlings infected with a F. pseudograminearum cytokinin reporter strain showed that the fungus specifically induces its cytokinin production in hyphae, which are in close association with the plant, suggestive of a function of Fusarium cytokinins during infection. Crown Copyright (C) 2019 Published by Elsevier Ltd on behalf of British Mycological Society. All rights reserved.

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KW - GFP

KW - Pathogen

KW - ESTIMATING DISEASE LOSSES

KW - CROWN ROT

KW - WHEAT

KW - DEOXYNIVALENOL

KW - IDENTIFICATION

KW - GRAMINEARUM

KW - GENES

KW - INFECTION

KW - VIRULENCE

KW - PATTERNS

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DO - 10.1016/j.funbio.2018.12.009

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JO - Fungal Biology

JF - Fungal Biology

SN - 0953-7562

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Blum A, Benfield AH, Sorensen JL, Nielsen MR, Bachleitner S, Studt L et al. Regulation of a novel Fusarium cytokinin in Fusarium pseudograminearum. Fungal Biology. 2019 Mar;123(3):255-266. https://doi.org/10.1016/j.funbio.2018.12.009