Recovery of aging-related size increase of skin epithelial cells: In vivo mouse and in vitro human study

I. Sokolov, N.V. Guz, Killugudi Iyer, A.L. Hewitt, N.A. Sokolov, J.S. Erlichman, C.D. Woodworth

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

© 2015 Sokolov et al. The size increase of skin epithelial cells during aging is well-known. Here we demonstrate that treatment of aging cells with cytochalasin B substantially decreases cell size. This decrease was demonstrated on a mouse model and on human skin cells in vitro. Six nude mice were treated by topical application of cytochalasin B on skin of the dorsal left midsection for 140 days (the right side served as control for placebo treatment). An average decrease in cell size of 56±16% resulted. A reduction of cell size was also observed on primary human skin epithelial cells of different in vitro age (passages from 1 to 8). A cell strain obtained from a pool of 6 human subjects was treated with cytochalasin B in vitro for 12 hours. We observed a decrease in cell size that became statistically significant and reached 20-40% for cells of older passage (6-8 passages) whereas no substantial change was observed for younger cells. These results may be important for understanding the aging processes, and for cosmetic treatment of aging skin.
Original languageEnglish
Pages (from-to)1-10
JournalPLoS One
Volume10
Issue number3
DOIs
Publication statusPublished - 2015

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