Reconstruction of the block matching profiles

N. Ketheesan, Silvana Gaudieri, C.S. Witt, Guan Tay, D.C. Townend, Frank Christiansen, R.L. Dawkins

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Block matching is a valuable tool for selecting donors for bone marrow transplantation. Identical, electrophoretic profiles of unrelated bone marrow donor-recipient pairs have been shown to be associated with long-term survival and a reduction of graft versus host disease (GVHD). This study was undertaken to determine the sequences of the PCR products which are generated. PCR products obtained with beta-block primers following the amplification of DNA extracted from cell lines homozygous for 7.1 and 8.1 ancestral haplotypes were cloned and sequenced. The PCR products were characterised and the beta block profiles reconstructed. The data indicate that the profiles consist of homoduplexes and heteroduplexes which are formed by the products of probably ? different sequence locations. Human Immunology GO, 171-176 (1999). (C) American Society for Histocompatibility and Immunogenetics, 1999. Published by Elsevier Science Inc.
Original languageEnglish
Pages (from-to)171-176
JournalHuman Immunology
Volume60
Issue number2
DOIs
Publication statusPublished - 1998

    Fingerprint

Cite this

Ketheesan, N., Gaudieri, S., Witt, C. S., Tay, G., Townend, D. C., Christiansen, F., & Dawkins, R. L. (1998). Reconstruction of the block matching profiles. Human Immunology, 60(2), 171-176. https://doi.org/10.1016/S0198-8859(98)00103-7