Recombinant monomeric CD40 ligand for delivering polymer particles to dendritic cells

R Noelle Palumbo, Lakshmi Nagarajan, Chun Wang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Dendritic cells (DCs) are considered the most efficient antigen-presenting cells and are therefore ideal targets for in vivo delivery of antigen for vaccines. We are investigating the strategy of using CD40 ligand (CD40L) as a targeting moiety because this protein has the potential to not only target DCs, but also stimulate cell maturation, leading to more potent immune responses. We have shown that a recombinant, monomeric CD40 ligand fusion protein conjugated to polystyrene micro- and nanoparticles led to significantly enhanced uptake by DCs in vitro. This enhancement was observed for particles of both sizes and in both a murine DC cell line and primary DCs. The uptake appeared to be specifically mediated by CD40L binding to CD40 expressed on DCs. Enhanced uptake of nanoparticles in draining lymph nodes of mice was not observed, however, 48 hours after subcutaneous injection. These findings suggest that CD40 ligand may be a potentially useful targeting moiety for delivery of particulate vaccines to DCs, and that further optimization of both CD40L and the polymer carriers is necessary to achieve efficacy in vivo.

Original languageEnglish
Pages (from-to)830-7
Number of pages8
JournalBiotechnology Progress
Volume27
Issue number3
DOIs
Publication statusPublished - 4 May 2011

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