TY - BOOK
T1 - Recombinant expression, purification and biophysical characterisation of virulence factors from Gram-negative pathogens
AU - Yalamanchili, Sampath Kumar
PY - 2019
Y1 - 2019
N2 - Endotoxin are complex molecules that provide Gram-negative pathogens with antibiotic resistance. They contain a hydrophobic anchor called lipid A. Lipid A biosynthesis enzymes UDP-2,3 diacylglucosamine hydrolase (LpxH) and Lipid A disaccharide synthase (LpxB) have been identified as drug targets. Another enzyme target of importance is cholesterolalpha-glycosyltransferase (CGT) from H. pylori, which synthesises glucosyl-cholesterol protecting the bacterium from changing environmental conditions. Recombinant expression and biophysical studies on these enzymes indicates they are conformationally stable and monodisperse while bioinformatic studies predicted the membrane interaction region for substrate recognition. These studies lay the foundation for future work focussed on structure-based drug design.
AB - Endotoxin are complex molecules that provide Gram-negative pathogens with antibiotic resistance. They contain a hydrophobic anchor called lipid A. Lipid A biosynthesis enzymes UDP-2,3 diacylglucosamine hydrolase (LpxH) and Lipid A disaccharide synthase (LpxB) have been identified as drug targets. Another enzyme target of importance is cholesterolalpha-glycosyltransferase (CGT) from H. pylori, which synthesises glucosyl-cholesterol protecting the bacterium from changing environmental conditions. Recombinant expression and biophysical studies on these enzymes indicates they are conformationally stable and monodisperse while bioinformatic studies predicted the membrane interaction region for substrate recognition. These studies lay the foundation for future work focussed on structure-based drug design.
KW - lipid A biosynthesis
KW - bacterial virulence factors
KW - peripheral membrane protein
KW - cholesterol glycosylation
KW - recombinant protein compression and purification
KW - biophysical characterisation of proteins
KW - enzymes
U2 - 10.26182/5d8d633600ecc
DO - 10.26182/5d8d633600ecc
M3 - Doctoral Thesis
ER -