Abstract
The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.
| Original language | English |
|---|---|
| Article number | 1903 |
| Journal | Scientific Reports |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Dec 2017 |
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Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia. / Mulroney, K. T.; Hall, J. M.; Huang, X.; Turnbull, E.; Bzdyl, N. M.; Chakera, A.; Naseer, U.; Corea, E. M.; Ellington, M. J.; Hopkins, K. L.; Wester, A. L.; Ekelund, O.; Woodford, N.; Inglis, T. J.J.
In: Scientific Reports, Vol. 7, No. 1, 1903, 01.12.2017.Research output: Contribution to journal › Article
TY - JOUR
T1 - Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia
AU - Mulroney, K. T.
AU - Hall, J. M.
AU - Huang, X.
AU - Turnbull, E.
AU - Bzdyl, N. M.
AU - Chakera, A.
AU - Naseer, U.
AU - Corea, E. M.
AU - Ellington, M. J.
AU - Hopkins, K. L.
AU - Wester, A. L.
AU - Ekelund, O.
AU - Woodford, N.
AU - Inglis, T. J.J.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.
AB - The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.
UR - http://www.scopus.com/inward/record.url?scp=85019403357&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-02009-3
DO - 10.1038/s41598-017-02009-3
M3 - Article
VL - 7
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 1903
ER -