Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia

K. T. Mulroney, J. M. Hall, X. Huang, E. Turnbull, N. M. Bzdyl, A. Chakera, U. Naseer, E. M. Corea, M. J. Ellington, K. L. Hopkins, A. L. Wester, O. Ekelund, N. Woodford, T. J.J. Inglis

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Abstract

The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.

Original languageEnglish
Article number1903
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

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Carbapenems
Klebsiella pneumoniae
Flow Cytometry
Workflow
Gram-Positive Bacteria
Anti-Bacterial Agents
Bacteria

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Mulroney, K. T. ; Hall, J. M. ; Huang, X. ; Turnbull, E. ; Bzdyl, N. M. ; Chakera, A. ; Naseer, U. ; Corea, E. M. ; Ellington, M. J. ; Hopkins, K. L. ; Wester, A. L. ; Ekelund, O. ; Woodford, N. ; Inglis, T. J.J. / Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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abstract = "The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.",
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Mulroney, KT, Hall, JM, Huang, X, Turnbull, E, Bzdyl, NM, Chakera, A, Naseer, U, Corea, EM, Ellington, MJ, Hopkins, KL, Wester, AL, Ekelund, O, Woodford, N & Inglis, TJJ 2017, 'Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia' Scientific Reports, vol. 7, no. 1, 1903. https://doi.org/10.1038/s41598-017-02009-3

Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia. / Mulroney, K. T.; Hall, J. M.; Huang, X.; Turnbull, E.; Bzdyl, N. M.; Chakera, A.; Naseer, U.; Corea, E. M.; Ellington, M. J.; Hopkins, K. L.; Wester, A. L.; Ekelund, O.; Woodford, N.; Inglis, T. J.J.

In: Scientific Reports, Vol. 7, No. 1, 1903, 01.12.2017.

Research output: Contribution to journalArticle

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