Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition

RI Griffiths; AS Whiteley; A.G. O'Donnell; MJ Bailey

doi:10.1128/AEM.66.12.5488-5491.2000

Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition

RI Griffiths, AS Whiteley, A.G. O'Donnell, MJ Bailey

Research output: Contribution to journal › Article

1094 Citations (Scopus)

Abstract

A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction, Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.

Original language	English
Pages (from-to)	5488-5491
Journal	Applied and Environmental Microbiology
Volume	66
DOIs	https://doi.org/10.1128/AEM.66.12.5488-5491.2000
Publication status	Published - 2000

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title = "Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition",

abstract = "A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction, Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.",

author = "RI Griffiths and AS Whiteley and A.G. O'Donnell and MJ Bailey",

year = "2000",

doi = "10.1128/AEM.66.12.5488-5491.2000",

language = "English",

volume = "66",

pages = "5488--5491",

journal = "Applied Environmental Microbiology",

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publisher = "American Society for Microbiology",

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T1 - Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition

AU - Griffiths, RI

AU - Whiteley, AS

AU - O'Donnell, A.G.

AU - Bailey, MJ

PY - 2000

Y1 - 2000

N2 - A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction, Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.

AB - A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction, Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.

U2 - 10.1128/AEM.66.12.5488-5491.2000

DO - 10.1128/AEM.66.12.5488-5491.2000

M3 - Article

VL - 66

SP - 5488

EP - 5491

JO - Applied Environmental Microbiology

JF - Applied Environmental Microbiology

SN - 0099-2240

ER -