Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition

RI Griffiths, AS Whiteley, A.G. O'Donnell, MJ Bailey

    Research output: Contribution to journalArticle

    1094 Citations (Scopus)

    Abstract

    A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction, Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.
    Original languageEnglish
    Pages (from-to)5488-5491
    JournalApplied and Environmental Microbiology
    Volume66
    DOIs
    Publication statusPublished - 2000

    Fingerprint Dive into the research topics of 'Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition'. Together they form a unique fingerprint.

    Cite this