Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori

W. Lonsdale; D. K. Maurya; M. Wajrak; C. Y. Tay; B. J. Marshall; K. Alameh

doi:10.1016/j.snb.2016.06.024

Rapid measurement of urease activity using a potentiometric RuO₂ pH sensor for detection of Helicobacter pylori

W. Lonsdale, D. K. Maurya, M. Wajrak, C. Y. Tay, B. J. Marshall, K. Alameh

Infection and Immunity

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

The feasibility of using a thin-film RuO₂ (ruthenium oxide) pH sensor for the detection of Helicobacter pylori is investigated. In particular, we demonstrate the ability of the sensor to measure urease activity between 1.0 and 50 U/mL in less than 30 s. The developed sensor exhibited a super-Nernstian response of 77.74 mV/pH and excellent reversibility. The pH sensor⿿s ability to detect H. pylori in real time is demonstrated, and found to be much faster than the rapid urease test.

Original language	English
Pages (from-to)	1305-1308
Number of pages	4
Journal	Sensors and Actuators, B: Chemical
Volume	242
DOIs	https://doi.org/10.1016/j.snb.2016.06.024
Publication status	Published - 1 Apr 2017

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10.1016/j.snb.2016.06.024

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1 Finished

Sir Macfarlane Burnet Fellowship - Marshall
Marshall, B.
National Health & Medical Research Council NHMRC
31/12/08 → 31/12/15
Project: Research

Cite this

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title = "Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori",

abstract = "The feasibility of using a thin-film RuO2 (ruthenium oxide) pH sensor for the detection of Helicobacter pylori is investigated. In particular, we demonstrate the ability of the sensor to measure urease activity between 1.0 and 50 U/mL in less than 30 s. The developed sensor exhibited a super-Nernstian response of 77.74 mV/pH and excellent reversibility. The pH sensor⿿s ability to detect H. pylori in real time is demonstrated, and found to be much faster than the rapid urease test.",

keywords = "H. pylori detection, pH sensor, R.F. sputtering, Ruthenium oxide, Urease activity",

author = "W. Lonsdale and Maurya, {D. K.} and M. Wajrak and Tay, {C. Y.} and Marshall, {B. J.} and K. Alameh",

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T1 - Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori

AU - Lonsdale, W.

AU - Maurya, D. K.

AU - Wajrak, M.

AU - Tay, C. Y.

AU - Marshall, B. J.

AU - Alameh, K.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - The feasibility of using a thin-film RuO2 (ruthenium oxide) pH sensor for the detection of Helicobacter pylori is investigated. In particular, we demonstrate the ability of the sensor to measure urease activity between 1.0 and 50 U/mL in less than 30 s. The developed sensor exhibited a super-Nernstian response of 77.74 mV/pH and excellent reversibility. The pH sensor⿿s ability to detect H. pylori in real time is demonstrated, and found to be much faster than the rapid urease test.

AB - The feasibility of using a thin-film RuO2 (ruthenium oxide) pH sensor for the detection of Helicobacter pylori is investigated. In particular, we demonstrate the ability of the sensor to measure urease activity between 1.0 and 50 U/mL in less than 30 s. The developed sensor exhibited a super-Nernstian response of 77.74 mV/pH and excellent reversibility. The pH sensor⿿s ability to detect H. pylori in real time is demonstrated, and found to be much faster than the rapid urease test.

KW - H. pylori detection

KW - pH sensor

KW - R.F. sputtering

KW - Ruthenium oxide

KW - Urease activity

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M3 - Article

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JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

ER -

Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori

Abstract

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Sir Macfarlane Burnet Fellowship - Marshall

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Rapid measurement of urease activity using a potentiometric RuO₂ pH sensor for detection of Helicobacter pylori