Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

C. Yan; Jason W. Schmidberger; F. Parmeggiani; S.A.  Hussain; N.J.  Turner; S.L.  Flitsch; P. Barran

doi:10.1039/c6an00617e

Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

C. Yan, Jason W. Schmidberger, F. Parmeggiani, S.A. Hussain, N.J. Turner, S.L. Flitsch, P. Barran

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

© The Royal Society of Chemistry 2016.The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and µL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.

Original language	English
Pages (from-to)	2351-2355
Number of pages	5
Journal	Analyst
Volume	141
Issue number	8
DOIs	https://doi.org/10.1039/c6an00617e
Publication status	Published - 21 Apr 2016
Externally published	Yes

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title = "Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry",

abstract = "{\textcopyright} The Royal Society of Chemistry 2016.The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and µL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.",

author = "C. Yan and Schmidberger, {Jason W.} and F. Parmeggiani and S.A. Hussain and N.J. Turner and S.L. Flitsch and P. Barran",

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AU - Yan, C.

AU - Schmidberger, Jason W.

AU - Parmeggiani, F.

AU - Hussain, S.A.

AU - Turner, N.J.

AU - Flitsch, S.L.

AU - Barran, P.

PY - 2016/4/21

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N2 - © The Royal Society of Chemistry 2016.The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and µL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.

AB - © The Royal Society of Chemistry 2016.The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and µL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.

U2 - 10.1039/c6an00617e

DO - 10.1039/c6an00617e

M3 - Article

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SN - 0003-2654

VL - 141

SP - 2351

EP - 2355

JO - Analyst

JF - Analyst

IS - 8

ER -

Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

Abstract

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