Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis

for the CASTLE Study team

doi:10.1111/ajo.13503

Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis

for the CASTLE Study team

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Mastitis is commonly experienced by breastfeeding women. While Staphylococcus aureus is usually implicated in infectious mastitis, coagulase-negative staphylococci (CoNS) are a possible alternative pathogen. This case-control study examined the role of CoNS in mastitis using isolates cultured from breast milk of 20 women with mastitis and 16 women without mastitis. Gene sequencing determined bacterial species, and random amplified polymorphic DNA (RAPD) analysis investigated strain-level variation. The majority of CoNS isolates were Staphylococcus epidermidis (182/199; 91%). RAPD analysis identified 33 unique S. epidermidis profiles, with no specific profile associated with mastitis cases.

Original language	English
Pages (from-to)	605-609
Number of pages	5
Journal	Australian and New Zealand Journal of Obstetrics and Gynaecology
Volume	62
Issue number	4
Early online date	2022
DOIs	https://doi.org/10.1111/ajo.13503
Publication status	Published - Aug 2022

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10.1111/ajo.13503Licence: CC BY-NC-ND

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title = "Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis",

abstract = "Mastitis is commonly experienced by breastfeeding women. While Staphylococcus aureus is usually implicated in infectious mastitis, coagulase-negative staphylococci (CoNS) are a possible alternative pathogen. This case-control study examined the role of CoNS in mastitis using isolates cultured from breast milk of 20 women with mastitis and 16 women without mastitis. Gene sequencing determined bacterial species, and random amplified polymorphic DNA (RAPD) analysis investigated strain-level variation. The majority of CoNS isolates were Staphylococcus epidermidis (182/199; 91%). RAPD analysis identified 33 unique S. epidermidis profiles, with no specific profile associated with mastitis cases.",

keywords = "case-control study, coagulase-negative staphylococci, mastitis, Staphylococcus epidermidis",

author = "{for the CASTLE Study team} and Meabh Cullinane and Lauren Scofield and Murray, {Gerald L.} and Payne, {Matthew S.} and Bennett, {Catherine M.} and Garland, {Suzanne M.} and Amir, {Lisa H.}",

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AU - Payne, Matthew S.

AU - Bennett, Catherine M.

AU - Garland, Suzanne M.

AU - Amir, Lisa H.

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Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis

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