TY - JOUR
T1 - Quantitative noninvasive angiography of the fovea centralis using speckle variance optical coherence tomography
AU - Mammo, Z.
AU - Balaratnasingam, Chandrakumar
AU - Yu, Paula
AU - Xu, J.
AU - Heisler, M.
AU - Mackenzie, P.
AU - Merkur, A.
AU - Kirker, A.
AU - Albiani, D.
AU - Bailey Freund, K.
AU - Sarunic, M.V.
AU - Yu, Dao-Yi
PY - 2015
Y1 - 2015
N2 - © 2015 The Association for Research in Vision and Ophthalmology, Inc. PURPOSE. To demonstrate the utility of speckle variance optical coherence tomography (svOCT), a noninvasive angiographic technique, for evaluating the foveal vasculature. METHODS. Twelve normal human eyes were imaged with svOCT (1060-nm, 100-kHz custombuilt system) and fluorescein angiography (FA; Topcon TRC-50DX with 5.0 megapixel resolution camera). Manual tracing techniques were used to quantify the foveal vasculature, including foveal avascular zone (FAZ) metrics (area, perimeter, greatest diameter, and lowest diameter). Reproducibility of these measurements was determined. The FAZ was imaged in 25 normal eyes using svOCT and 15 donor eyes using confocal scanning laser microscopy. Retinal capillary plexuses in donor eyes were perfusion-labeled with phalloidin conjugated to Alexa Fluor 546. RESULTS. Speckle variance OCT is able to stratify the foveal circulation into inner and deep capillary plexuses as well as reliably quantify and assess the morphometric dimensions of the human FAZ. Capillary density measurements were significantly greater in svOCT than FA (31.2 ± 1.6% vs. 19.3 ± 1.9% of total tissue area; P <0.001). Measurements were highly reproducible (all P > 0.366). All FAZ metrics were significantly lower in histology than svOCT (all P <0.001). CONCLUSIONS. Speckle variance OCT permits precise, reproducible, and noninvasive visualization of the human foveal vasculature. Speckle variance OCT may become an important adjunct in evaluating patients with retinal vascular diseases.
AB - © 2015 The Association for Research in Vision and Ophthalmology, Inc. PURPOSE. To demonstrate the utility of speckle variance optical coherence tomography (svOCT), a noninvasive angiographic technique, for evaluating the foveal vasculature. METHODS. Twelve normal human eyes were imaged with svOCT (1060-nm, 100-kHz custombuilt system) and fluorescein angiography (FA; Topcon TRC-50DX with 5.0 megapixel resolution camera). Manual tracing techniques were used to quantify the foveal vasculature, including foveal avascular zone (FAZ) metrics (area, perimeter, greatest diameter, and lowest diameter). Reproducibility of these measurements was determined. The FAZ was imaged in 25 normal eyes using svOCT and 15 donor eyes using confocal scanning laser microscopy. Retinal capillary plexuses in donor eyes were perfusion-labeled with phalloidin conjugated to Alexa Fluor 546. RESULTS. Speckle variance OCT is able to stratify the foveal circulation into inner and deep capillary plexuses as well as reliably quantify and assess the morphometric dimensions of the human FAZ. Capillary density measurements were significantly greater in svOCT than FA (31.2 ± 1.6% vs. 19.3 ± 1.9% of total tissue area; P <0.001). Measurements were highly reproducible (all P > 0.366). All FAZ metrics were significantly lower in histology than svOCT (all P <0.001). CONCLUSIONS. Speckle variance OCT permits precise, reproducible, and noninvasive visualization of the human foveal vasculature. Speckle variance OCT may become an important adjunct in evaluating patients with retinal vascular diseases.
U2 - 10.1167/iovs.15-16773
DO - 10.1167/iovs.15-16773
M3 - Article
C2 - 26237197
SN - 0146-0404
VL - 56
SP - 5074
EP - 5086
JO - Investigative Ophthalmology & Visual Science (IOVS)
JF - Investigative Ophthalmology & Visual Science (IOVS)
IS - 9
ER -
