Quantitation of glutaminase mRNA in enterocytes using competitive RT-PCR

S-E. Kong, Kathy Heel, John Hall, Rosalie Mccauley

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The concentration of mRNA within the intestinal mucosa is usually measured by either Northern blot analysis or semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). However, these methods are limited by a lack of valid internal controls, low sensitivity, and large differences in the concentration of the internal control and target gene. The authors present an alternative method using competitive RT-PCR to measure glutaminase mRNA in isolated enterocytes. (C) 1998 Academic Press.
Original languageEnglish
Pages (from-to)339-341
JournalMolecular and Cellular Probes
Volume12
DOIs
Publication statusPublished - 1998

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