QTLs for stomatal and photosynthetic traits related to salinity tolerance in barley

Xiaohui Liu; Yun Fan; Michelle Mak; Mohammad Babla; Paul Holford; Feifei Wang; Guang Chen; Grace Scott; Gang Wang; Sergey Shabala; Meixue Zhou; Zhong Hua Chen

doi:10.1186/s12864-016-3380-0

QTLs for stomatal and photosynthetic traits related to salinity tolerance in barley

Xiaohui Liu, Yun Fan, Michelle Mak, Mohammad Babla, Paul Holford, Feifei Wang, Guang Chen, Grace Scott, Gang Wang, Sergey Shabala, Meixue Zhou, Zhong Hua Chen

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Background: Stomata regulate photosynthesis and transpiration, and these processes are critical for plant responses to abiotic stresses such as salinity. A barley double haploid population with 108 lines derived from a cross between CM72 (salt-tolerant) and Gairdner (salt-sensitive) was used to detect quantitative trait loci (QTLs) associated with stomatal and photosynthetic traits related to salinity tolerance. Results: A total of 11 significant QTLs (LOD > 3.0) and 11 tentative QTLs (2.5 < LOD < 3.0) were identified. These QTLs are distributed on all the seven chromosomes, except 5H and explain 9.5-17.3% of the phenotypic variation. QTLs for biomass, intercellular CO₂ concentration, transpiration rate and stomatal conductance under control conditions co-localised together. A QTL for biomass also co-located with one for transpiration rate under salinity stress. A linkage was found between stomatal pore area and gas exchange. A QTL for salinity tolerance also co-localised with QTLs for grain yield and biomass on chromosome 3H. Based on the draft barley genome, the candidate genes for salinity tolerance at this locus are proposed. Conclusions: The lack of major QTLs for gas exchange and stomatal traits under control and saline conditions indicates a complex relationship between salinity and leaf gas exchange due to the fact that these complex quantitative traits are under the control of multiple genes.

Original language	English
Article number	9
Journal	BMC Genomics
Volume	18
Issue number	1
DOIs	https://doi.org/10.1186/s12864-016-3380-0
Publication status	Published - 3 Jan 2017
Externally published	Yes

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@article{d340cd6551ec44f6bda8edf29f906661,

title = "QTLs for stomatal and photosynthetic traits related to salinity tolerance in barley",

abstract = "Background: Stomata regulate photosynthesis and transpiration, and these processes are critical for plant responses to abiotic stresses such as salinity. A barley double haploid population with 108 lines derived from a cross between CM72 (salt-tolerant) and Gairdner (salt-sensitive) was used to detect quantitative trait loci (QTLs) associated with stomatal and photosynthetic traits related to salinity tolerance. Results: A total of 11 significant QTLs (LOD > 3.0) and 11 tentative QTLs (2.5 < LOD < 3.0) were identified. These QTLs are distributed on all the seven chromosomes, except 5H and explain 9.5-17.3% of the phenotypic variation. QTLs for biomass, intercellular CO2 concentration, transpiration rate and stomatal conductance under control conditions co-localised together. A QTL for biomass also co-located with one for transpiration rate under salinity stress. A linkage was found between stomatal pore area and gas exchange. A QTL for salinity tolerance also co-localised with QTLs for grain yield and biomass on chromosome 3H. Based on the draft barley genome, the candidate genes for salinity tolerance at this locus are proposed. Conclusions: The lack of major QTLs for gas exchange and stomatal traits under control and saline conditions indicates a complex relationship between salinity and leaf gas exchange due to the fact that these complex quantitative traits are under the control of multiple genes.",

keywords = "Gas exchange, Guard cell, Hordeum vulgare L, QTL mapping, Stomatal regulation",

author = "Xiaohui Liu and Yun Fan and Michelle Mak and Mohammad Babla and Paul Holford and Feifei Wang and Guang Chen and Grace Scott and Gang Wang and Sergey Shabala and Meixue Zhou and Chen, {Zhong Hua}",

note = "Funding Information: We thank Jennie Nelson, Linda Westmoreland, Elizabeth Kabanoff, Sumedha Dharmaratne, Rosemary Freeman, Renee Smith and Honglang Duan for their technical assistance. This work was supported by the Australian Department of Agriculture and an Australian Research Council DECRA (DE140101143) to Zhong-Hua Chen. Meixue Zhou and Sergey Shabala were supported by the funding from the Grains Research and Development Corporation. Xiaohui Liu was a recipient of China Scholarship Council award. Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = jan,

day = "3",

doi = "10.1186/s12864-016-3380-0",

language = "English",

volume = "18",

journal = "BMC Genomics",

issn = "1471-2164",

publisher = "BioMed Central",

number = "1",

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TY - JOUR

T1 - QTLs for stomatal and photosynthetic traits related to salinity tolerance in barley

AU - Liu, Xiaohui

AU - Fan, Yun

AU - Mak, Michelle

AU - Babla, Mohammad

AU - Holford, Paul

AU - Wang, Feifei

AU - Chen, Guang

AU - Scott, Grace

AU - Wang, Gang

AU - Shabala, Sergey

AU - Zhou, Meixue

AU - Chen, Zhong Hua

N1 - Funding Information: We thank Jennie Nelson, Linda Westmoreland, Elizabeth Kabanoff, Sumedha Dharmaratne, Rosemary Freeman, Renee Smith and Honglang Duan for their technical assistance. This work was supported by the Australian Department of Agriculture and an Australian Research Council DECRA (DE140101143) to Zhong-Hua Chen. Meixue Zhou and Sergey Shabala were supported by the funding from the Grains Research and Development Corporation. Xiaohui Liu was a recipient of China Scholarship Council award. Publisher Copyright: © 2017 The Author(s).

PY - 2017/1/3

Y1 - 2017/1/3

N2 - Background: Stomata regulate photosynthesis and transpiration, and these processes are critical for plant responses to abiotic stresses such as salinity. A barley double haploid population with 108 lines derived from a cross between CM72 (salt-tolerant) and Gairdner (salt-sensitive) was used to detect quantitative trait loci (QTLs) associated with stomatal and photosynthetic traits related to salinity tolerance. Results: A total of 11 significant QTLs (LOD > 3.0) and 11 tentative QTLs (2.5 < LOD < 3.0) were identified. These QTLs are distributed on all the seven chromosomes, except 5H and explain 9.5-17.3% of the phenotypic variation. QTLs for biomass, intercellular CO2 concentration, transpiration rate and stomatal conductance under control conditions co-localised together. A QTL for biomass also co-located with one for transpiration rate under salinity stress. A linkage was found between stomatal pore area and gas exchange. A QTL for salinity tolerance also co-localised with QTLs for grain yield and biomass on chromosome 3H. Based on the draft barley genome, the candidate genes for salinity tolerance at this locus are proposed. Conclusions: The lack of major QTLs for gas exchange and stomatal traits under control and saline conditions indicates a complex relationship between salinity and leaf gas exchange due to the fact that these complex quantitative traits are under the control of multiple genes.

AB - Background: Stomata regulate photosynthesis and transpiration, and these processes are critical for plant responses to abiotic stresses such as salinity. A barley double haploid population with 108 lines derived from a cross between CM72 (salt-tolerant) and Gairdner (salt-sensitive) was used to detect quantitative trait loci (QTLs) associated with stomatal and photosynthetic traits related to salinity tolerance. Results: A total of 11 significant QTLs (LOD > 3.0) and 11 tentative QTLs (2.5 < LOD < 3.0) were identified. These QTLs are distributed on all the seven chromosomes, except 5H and explain 9.5-17.3% of the phenotypic variation. QTLs for biomass, intercellular CO2 concentration, transpiration rate and stomatal conductance under control conditions co-localised together. A QTL for biomass also co-located with one for transpiration rate under salinity stress. A linkage was found between stomatal pore area and gas exchange. A QTL for salinity tolerance also co-localised with QTLs for grain yield and biomass on chromosome 3H. Based on the draft barley genome, the candidate genes for salinity tolerance at this locus are proposed. Conclusions: The lack of major QTLs for gas exchange and stomatal traits under control and saline conditions indicates a complex relationship between salinity and leaf gas exchange due to the fact that these complex quantitative traits are under the control of multiple genes.

KW - Gas exchange

KW - Guard cell

KW - Hordeum vulgare L

KW - QTL mapping

KW - Stomatal regulation

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U2 - 10.1186/s12864-016-3380-0

DO - 10.1186/s12864-016-3380-0

M3 - Article

C2 - 28049416

AN - SCOPUS:85010211610

SN - 1471-2164

VL - 18

JO - BMC Genomics

JF - BMC Genomics

IS - 1

M1 - 9

ER -

QTLs for stomatal and photosynthetic traits related to salinity tolerance in barley

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