Provenance modulates sensitivity of stored seeds of the Australian native grass Neurachne alopecuroidea to temperature and moisture availability

Friday Gray, Anne Cochrane, Pieter Poot

Research output: Contribution to journalArticle

Abstract

In the present study we assessed the sensitivity of stored seeds of the common grass Neurachne alopecuroidea R.Br. from south-western Australian sources to varying temperature and moisture conditions as a tool to anticipate potential adaptability of seeds to climate change. Weexamined among-population germination responses, focusing on germination of excised seeds to overcome possible dormancy imparted by the lemma and palea. We hypothesised that temperature above and below the optimum and low moisture potentials would adversely affect germination, and that conditions for successful germination would be associated with the local climate at each seed source site. Experiment 1 used a bi-directional temperature gradient plate to measure responses to constant and alternating temperatures (5-40 degrees C). Experiment 2 examined responses to moisture availability using polyethylene glycol (PEG 8000) solutions at different temperatures. Temperature optima varied among populations with significant reductions in germination occurring only below 15 degrees C. Germination speed and success declined with decreasing moisture availability, with greater impact at higher temperatures. Significant population variation was observed. Tolerance to temperature and moisture availability was higher than expected and some of this variation suggests adaptation to local climates across the species Western Australian distribution. We discuss these results in the context of seed use in restoration and global warming.

Original languageEnglish
Pages (from-to)106-115
Number of pages10
JournalAustralian Journal of Botany
Volume67
Issue number2
DOIs
Publication statusPublished - 2019

Cite this

@article{8d00c387b2c84f1baa9f3a49c16b6826,
title = "Provenance modulates sensitivity of stored seeds of the Australian native grass Neurachne alopecuroidea to temperature and moisture availability",
abstract = "In the present study we assessed the sensitivity of stored seeds of the common grass Neurachne alopecuroidea R.Br. from south-western Australian sources to varying temperature and moisture conditions as a tool to anticipate potential adaptability of seeds to climate change. Weexamined among-population germination responses, focusing on germination of excised seeds to overcome possible dormancy imparted by the lemma and palea. We hypothesised that temperature above and below the optimum and low moisture potentials would adversely affect germination, and that conditions for successful germination would be associated with the local climate at each seed source site. Experiment 1 used a bi-directional temperature gradient plate to measure responses to constant and alternating temperatures (5-40 degrees C). Experiment 2 examined responses to moisture availability using polyethylene glycol (PEG 8000) solutions at different temperatures. Temperature optima varied among populations with significant reductions in germination occurring only below 15 degrees C. Germination speed and success declined with decreasing moisture availability, with greater impact at higher temperatures. Significant population variation was observed. Tolerance to temperature and moisture availability was higher than expected and some of this variation suggests adaptation to local climates across the species Western Australian distribution. We discuss these results in the context of seed use in restoration and global warming.",
keywords = "climate change, germination, moisture availability, Poaceae, seed bank, temperature, Western Australia, POLYETHYLENE-GLYCOL, CLIMATE-CHANGE, POPULATION VARIATION, ALLIES POACEAE, GERMINATION, DORMANCY, STRESS, C-3, TRAITS",
author = "Friday Gray and Anne Cochrane and Pieter Poot",
year = "2019",
doi = "10.1071/BT18240",
language = "English",
volume = "67",
pages = "106--115",
journal = "Australian Journal of Botany",
issn = "0067-1924",
publisher = "CSIRO Publishing",
number = "2",

}

TY - JOUR

T1 - Provenance modulates sensitivity of stored seeds of the Australian native grass Neurachne alopecuroidea to temperature and moisture availability

AU - Gray, Friday

AU - Cochrane, Anne

AU - Poot, Pieter

PY - 2019

Y1 - 2019

N2 - In the present study we assessed the sensitivity of stored seeds of the common grass Neurachne alopecuroidea R.Br. from south-western Australian sources to varying temperature and moisture conditions as a tool to anticipate potential adaptability of seeds to climate change. Weexamined among-population germination responses, focusing on germination of excised seeds to overcome possible dormancy imparted by the lemma and palea. We hypothesised that temperature above and below the optimum and low moisture potentials would adversely affect germination, and that conditions for successful germination would be associated with the local climate at each seed source site. Experiment 1 used a bi-directional temperature gradient plate to measure responses to constant and alternating temperatures (5-40 degrees C). Experiment 2 examined responses to moisture availability using polyethylene glycol (PEG 8000) solutions at different temperatures. Temperature optima varied among populations with significant reductions in germination occurring only below 15 degrees C. Germination speed and success declined with decreasing moisture availability, with greater impact at higher temperatures. Significant population variation was observed. Tolerance to temperature and moisture availability was higher than expected and some of this variation suggests adaptation to local climates across the species Western Australian distribution. We discuss these results in the context of seed use in restoration and global warming.

AB - In the present study we assessed the sensitivity of stored seeds of the common grass Neurachne alopecuroidea R.Br. from south-western Australian sources to varying temperature and moisture conditions as a tool to anticipate potential adaptability of seeds to climate change. Weexamined among-population germination responses, focusing on germination of excised seeds to overcome possible dormancy imparted by the lemma and palea. We hypothesised that temperature above and below the optimum and low moisture potentials would adversely affect germination, and that conditions for successful germination would be associated with the local climate at each seed source site. Experiment 1 used a bi-directional temperature gradient plate to measure responses to constant and alternating temperatures (5-40 degrees C). Experiment 2 examined responses to moisture availability using polyethylene glycol (PEG 8000) solutions at different temperatures. Temperature optima varied among populations with significant reductions in germination occurring only below 15 degrees C. Germination speed and success declined with decreasing moisture availability, with greater impact at higher temperatures. Significant population variation was observed. Tolerance to temperature and moisture availability was higher than expected and some of this variation suggests adaptation to local climates across the species Western Australian distribution. We discuss these results in the context of seed use in restoration and global warming.

KW - climate change

KW - germination

KW - moisture availability

KW - Poaceae

KW - seed bank

KW - temperature

KW - Western Australia

KW - POLYETHYLENE-GLYCOL

KW - CLIMATE-CHANGE

KW - POPULATION VARIATION

KW - ALLIES POACEAE

KW - GERMINATION

KW - DORMANCY

KW - STRESS

KW - C-3

KW - TRAITS

U2 - 10.1071/BT18240

DO - 10.1071/BT18240

M3 - Article

VL - 67

SP - 106

EP - 115

JO - Australian Journal of Botany

JF - Australian Journal of Botany

SN - 0067-1924

IS - 2

ER -