Mautia Hill, situated in the Mozambique Belt of East Africa, is a locality famous for its unique high-pressure white-schist mineral assemblages. The rocks are located in a complex regional tectonic setting that is critical to understanding the amalgamation history of Gondwana. The timing of the Pan-African Gondwana collision in this region is still a topic of considerable debate, especially because the age of the Mautia high-pressure metamorphism/metasomatism has yet to be established. We have extracted detrital zircons with extensive low-U overgrowths and dated them using the U-Pb SHRIMP method. Detrital zircon cores indicate that the sediments came from a region identical to the adjacent Tanzania Craton and adjacent Western Mozambique Belt. One detrital grain has a Pb-207/Pb-206 age of ca. 3.5 Ga and is the oldest dated zircon in the region. The zircon rims provide 207-corrected Pb-206/U-238 and Pb-208/ Th-232 weighted mean ages of and Ma, respectively, dating the age of collision between the Tanzania 549 +/- 41 and 535 +/- 40 Craton and Madagascar-India during the assembly of Gondwana. This new age indicates that collisional orogenesis in this part of the East African Orogen occurred in a similar time frame as in many other parts of Gondwana, including South America, southern Africa, India, Australia, and Antarctica, but later than a similar to 640-Ma age previously attributed to the collision in this area by some authors.