Proteomic identification of extracellular proteins regulated by the Gna1 Gα subunit in Stagonospora nodorum

K.C. Tan, J.L. Heazlewood, Harvey Millar, R.P. Oliver, P.S. Solomon

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The fungus Stagonospora nodorum is the causal agent of stagonospora nodorum blotch (syn.leaf and glume blotch) disease of wheat. The Gna1-encoded Ga protein is an important signaltransduction component in the fungus, which is required for full pathogenicity, sporulationand extracellular depolymerase production. In this study, we sought to gaina better understanding of defects associated with the gna1 mutant by using twodimensionalgel electrophoresis to analyse the extracellular proteome for differences tothe wildtype. Mass spectrometry analysis of altered abundant protein spots and peptidematching to the Stagonospora nodorum genome database have led to the identification ofgenes implicated in cell wall degradation, proteolysis, RNA hydrolysis and aromatic compoundmetabolism. In addition, quantitative RT-PCR has demonstrated that some of theencoding genes showed differential expression throughout host infection. Implicationsof these proteins and their corresponding genes in fungal virulence are discussed. ©2009 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)523-531
JournalMycological Research
Volume113
Issue number5
DOIs
Publication statusPublished - 2009

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