Proteomic analysis of lupin seed proteins to identify conglutin β as an allergen, Lup an 1

Danica Goggin, G. Mir, Wendy-Anne Smith, M. Stuckey, P.M.C. Smith

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Lupin products may be valuable as human foods because of their high protein content and potential anticholesterolemic properties. However, a small percentage of the population is allergic to lupin. In this study, we use in vitro IgE binding and mass spectrometry to identify conglutin beta, a major storage protein, as an allergen in seeds of Lupinus angustifolius and Lupinus albus. Purification of conglutin beta from L. angustifolius flour confirmed that serum IgE binds to this protein. Where IgE in sera recognized lupin proteins on Western blots, it recognized conglutin beta, suggesting this protein is a major allergen for lupin. The L. angustifolius conglutin beta allergen has been designated Lup an 1 by the International Union of Immunological Societies (IUIS) allergen nomenclature subcommittee.
Original languageEnglish
Pages (from-to)6370-6377
JournalJournal of Agricultural and Food Chemistry
Volume56
Issue number15
DOIs
Publication statusPublished - 2008

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