Proteomic Analysis of in Vitro Induced Fluoroquinolone Resistant Staphylococcus aureus

Van Chi Thai, Kim Phuong Uyen Le, Thi Thu Hoai Nguyen

Research output: Chapter in Book/Conference paperConference paperpeer-review

Abstract

Widespread use of antimicrobial agents in bacterial infection treatment has led to the outbreak of large number of resistant strains. In this study, we exposed Staphylococcus aureus (S. aureus) ATCC 29213 to ciprofloxacin (CIP), ofloxacin (OFL) or levofloxacin (LEV) at sub-minimum inhibitory concentrations (sub-MIC) for 12 days to obtain resistant strains and cultured for 10 days without antibiotics to obtain revertant strains. The seven protein samples from the initial strain, the 3 resistant and 3 revertant strains were extracted via sonication and cleaned by using 2D clean-up kit (GE Healthcare, USA). Protein samples were then quantitated by using RCDC protein assay kit (Bio-Rad, USA). Two-dimensional polyacrylamide gel electrophoresis (2D- PAGE) was performed at pH range 3–10. Briefly, the extracted protein of FQ- exposed strains with resistant phenotypes could then be compared with initial S. aureus ATCC 29213 in order identify protein spots associated with antibiotic resistance phenotype. After performing of 2D-PAGE and analyzing the 2D gels by using DECOCON Delta 2D software (Delta Decodon GmbH, Germany), the change in the expression of proteins including both up- and down- regulated ones in different FQ- exposed strains could be observed. Those protein spots seemed well correlated to resistance phenotypes.
Original languageEnglish
Title of host publication6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6)
EditorsToi Vo Van, Thanh An Nguyen Le, Thang Nguyen Duc
Place of PublicationSingapore
Pages295-299
Number of pages5
ISBN (Electronic)9789811043611
DOIs
Publication statusPublished - 2018
Externally publishedYes
Event6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6) - Ho Chi Minh, Viet Nam
Duration: 27 Jun 201629 Jun 2016

Publication series

NameIFMBE Proceedings
Volume63
ISSN (Print)1680-0737

Conference

Conference6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6)
Abbreviated titleBME 2016
Country/TerritoryViet Nam
CityHo Chi Minh
Period27/06/1629/06/16

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